Comparative Evaluation of the Proliferative and Epithelial Mesenchymal Transition Characteristics in Oral Squamous Cell Carcinoma: A Case Control study

Jerlin Margret, F (2020) Comparative Evaluation of the Proliferative and Epithelial Mesenchymal Transition Characteristics in Oral Squamous Cell Carcinoma: A Case Control study. Masters thesis, Rajas Dental College and Hospital, Tirunelveli.


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BACKGROUND: Oral squamous cell carcinoma accounts for 90% of all oral cancer. It is a lethal disease having the survival rate of 5 years. The risk factors for OSCC are smoking, tobacco chewing, alcohol consumption and certain virus. Cancer develops as a multistep process with genetic changes. The poor prognosis is due to late diagnosis, recurrence, and metastasis. Early diagnosis and proper treatment can reduce the morbidity. Increased cellular proliferation, increased expression of cancer stem cells and loss of intercellular adhesion molecules are the important features of the tissue when it is transforming into cancer. AIM OF THE STUDY: The aim of this study is to assess the proliferative potential and EMT in OSCC compared to controls. OBJECTIVES: To evaluate the expression, of ki-67, Oct4and E-cadherin in cases and controls. Comparing the expression of Ki-67, Oct4 and E-cadherin, between cases and controls. Correlating the expression of Ki-67, Oct4and E-cadherin between the histopathological grades among the cases. MATERIALS AND METHODS: Paraffin embedded tissue sections of OSCC (n =30). They include 10 well differentiated OSCC and 10 moderately differentiated OSCC and 10 poorly differentiated OSCC and normal mucosa (n =10) were confirmed histopathologically. The sections were stained with antibodies against Ki-67, Oct4 and E-cadherin and positive staining were graded. RESULTS: In Ki-67 there was a statistically significant result in cases when compared to controls. Comparing between well, moderate and poorly differentiated OSCC, there was a statistically significant increase in mean labelling index as the grades were increasing. In analysing Oct4 the difference was not statistically significant when cases were compared to controls. In case of E-cadherin, there was a statistically significant decrease in E-cadherin among cases compared to controls. There was a decrease in the expression of E- cadherin as the grades of OSCC were increasing. The difference was not statistically significant. CONCLUSION: This study concludes that Ki-67 can be used as a prognostic marker in OSCC. E-cadherin can be used to analyse invasive phenotypes of OSCC. Oct4 expression is less conclusive in OSCC.

Item Type: Thesis (Masters)
Additional Information: 241721552
Uncontrolled Keywords: Ki-67, E-cadherin, Oct4, Oral Squamous Cell Carcinoma, Immunohistochemistry.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 24 Feb 2021 16:35
Last Modified: 26 Feb 2021 01:24

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