Preetha, S (2020) Expression of Aldehyde Dehydrogenase 1 (ALDH 1) and CD144 in Non-habit associated Oral Squamous Cell Carcinoma. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Cancer stem cells are tumour cells that have the capacity of self-renewal, the potential to develop into any cell in the overall tumour population and the proliferative ability to drive continued expansion of the population of malignant cells. Aldehyde dehydrogenase 1 (ALDH1) is a cell surface marker for cancer stem cells. ALDH1 is involved in the conversion of retinol to retinoic acid and catalyses the oxidation of intercellular aldehyde metabolites into carboxylic acid. Vascular Endothelial-Cadherin (VE-Cadherin; CD144) is an adhesion molecule that promotes cell-to-cell interaction. CD144 also controls the cohesion and organization of the intercellular junctions. Overexpression level of VE-Cadherin enhances the cancer neovascularization, growth, and progression. AIM AND OBJECTIVES: To evaluate the expression of ALDH 1 and CD144 in patients with non-habit associated OralSquamous Cell Carcinoma by Immunohistochemistry(IHC). MATERIALS AND METHODS: Immunohistochemical detection of ALDH1 was done using polyclonal antibody and Poly Excel HRP/DABTMchromogen detection system on 37formalin fixed paraffin embedded tissue samples, which included non-habit associated Oral Squamous Cell Carcinoma(OSCC; n=20) and the expression was compared with that of normal mucosa(n=17). Immunohistochemical detection of CD144 was done using polyclonal antibody and Poly ExcelHRP/DAB chromogen detection system on 37formalin fixed paraffin embedded tissue samples,which included non-habit associated Oral Squamous Cell Carcinoma(OSCC; n=20) and the expression was compared with that of normal mucosa(n=17). RESULTS: The positivity of CD144 expression was higher in OSCC when compared with normal mucosa. The connective tissue also showed positively stained vascular channels, cells around the vascular channels and keratin pearls. ALDH1 positivity was expressed more in normal mucosa when compared to OSCC. The connective tissue showed ALDH1 positively stained blood vessels, keratin pearls and muscle. CONCLUSION: When comparing the expression of CD144 and ALDH1 between the two groups, CD144 was increased in OSCC (Group I) and decreased in normal mucosa (Group II). ALDH1 was increased in normal mucosa and decreased in OSCC. The decreased expression of ALDH1 in OSCC can be due to the lower levels of acetaldehyde during alcohol metabolism in non-alcoholics and the biological behaviour difference between smokers and alcoholics when compared to non-smokers and non-alcoholics.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241721153 |
| Uncontrolled Keywords: | ALDH1, CD144, Oral Squamous Cell Carcinoma(OSCC). |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Feb 2021 16:27 |
| Last Modified: | 26 Feb 2021 01:10 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14478 |
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