Ashwin Andrews, E (2020) Autologous Gingival Fibroblast Culture on Commercially Available Collagen Membrane. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Gingival fibroblasts play an important role in soft tissue healing and regeneration. Gingival tissue is a source for mesenchymal stem cells in tissue engineering. AIM OF THE STUDY: To assess the viability of gingival fibroblast before and after seeding on to collagen membrane. MATERIALS AND METHOD: Isolation of gingival fibroblasts was done for 5 samples. Morphology and growth characteristics was observed using a phase contrast microscope and viability was assessed using trypan blue and MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay. RESULTS: Five successful cultures yielded sufficient cells for characterization. Among five, growth characteristics and morphology were assessed for one sample. The population doubling time (PDT) and the seeding efficiency was 2.608 and 72.6% respectively. The gingival fibroblasts showed 94% viability before seeding on to collagen membrane. The MTT assay which was done after seeding on to collagen membrane suggested no considerable difference in the viability of fibroblasts. At One hour the gingival fibroblast adhered to the collagen membrane showed a rounded morphology. After 24 hours the gingival fibroblast attached to the collagen membrane showed a spindle shaped morphology. CONCLUSION: The gingival fibroblasts were isolated from gingival connective tissue of five different samples. The growth characteristics and phenotype of gingival fibroblasts was assessed for one sample. The gingival fibroblasts were assessed before and after seeding on to collagen membrane and were found to be viable.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241721151 |
| Uncontrolled Keywords: | Gingival fibroblasts, Collagen membrane, viability test. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Feb 2021 16:18 |
| Last Modified: | 26 Feb 2021 00:59 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14476 |
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