Anu Priya, G (2020) Evaluation of Anti-Bacterial Efficacy of Zirconium Oxide Nanoparticles (ZrO2NPs) against Streptococcus Mutans and Enterococcus Faecalis: An In Vitro study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
AIM OF THE STUDY: To evaluate the anti-bacterial efficacy of Zirconium Oxide Nanoparticles (ZrO2 NPs) against Streptococcus mutans and Enterococcus faecalis. METHODOLOGY: Standard ATCC strains of Streptococcus mutans (ATCC 25175) and Enterococcus faecalis (ATCC 29212) were procured from Sigma Aldrich and sub-cultured onto Brain Heart Infusion Agar (BHIA) plate at 37 0c for 24 hours. The strains were tested for their sensitivity to ZrO2 NPs at various dilutions (100mg/mL, 75mg/mL, 50mg/mL, 25mg/mL) using Agar well diffusion method. The minimum concentration of ZrO2 NPs to inhibit 99.9% growth of S.mutans and E. faecalis was determined by the standard methods (Broth Microdilution) of Clinical and Laboratory Standards Institute (CLSI) guidelines. Following which the ability of ZrO2 NPs to disrupt the biofilms formed by S. mutans and E. faecalis was evaluated by serial dilutions of ZrO2 NPs to determine the Minimum Biofilm Eradication Concentration. RESULTS: ZrO2 NPs exhibited clear zones of inhibition of 12mm and 15mm at a concentration of 100mg/mL against S. mutans and E. faecalis in the agar wells respectively. Similarly, ZrO2 NPs also disrupted the strongly adherent biofilms formed by S. mutans and E. faecalis at a concentration above 22μg/mL in the Broth microdilution method. CONCLUSION: The present study concluded that ZrO2 NPs have potential anti- bacterial activity against both S. mutans and E. faecalis and are also capable of eradicating the biofilms formed by them demonstrating the anti- biofilm property of ZrO2 NPs.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241717153 |
| Uncontrolled Keywords: | Zirconium Oxide Nanoparticles (ZrO2 NPs), Streptococcus mutans, Enterococcus faecalis, Anti- Microbial Susceptability testing, Broth Microdilution (MIC and MBEC), Dental Caries, Secondary endodontic infections. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Subramani R |
| Date Deposited: | 22 Feb 2021 16:58 |
| Last Modified: | 23 Feb 2021 02:05 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14408 |
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