Abirami, T S (2020) Association of Subgingival Colonization of Candida Species with the Severity of Chronic Periodontitis in Type 2 Newly Diagnosed Diabetic and Non- Diabetic Patients: A Case Control study. Masters thesis, Chettinad Dental College & Research Institute, Kanchipuram.
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Abstract
INTRODUCTION: Periodontitis is an inflammatory disease of the supporting tissues of the teeth caused by specific or groups of microorganisms, resulting in progressive destruct ion of the periodontal ligament and alveolar bone with pocket format ion, recession or both. It is a ubiquitous disease with multi factorial etiology namely the microbial, genetic, systemic, environmental and immunological factors. The microbial factors of periodontal disease have been the focus of intense investigation for several decades and were justified by their bacterial role. 3 But the changes in the paradigms over years impacted the study of the microbiota associated with etiology and pathogenesis of periodontal diseases. AIM AND OBJECTIVES: The aim of the study was to analyze the association of subgingival colonization of Candida species in stage II and stage III periodontitis patients with and without type 2 newly diagnosed diabetes mellitus. MATERIALS AND METHODS: This case control study comprised of 500 participants who were divided into 3 groups. Group 1 included 100 healthy patients with Dental biofilm induced gingivitis. Group 2 comprised of 200 stage II periodontitis patients, categorized into 100 patients without diabetes mellitus (Group 2A) and 100 patients with type 2 newly diagnosed diabetes mellitus (Group 2B). Group 3 comprised of 200 stage III periodontitis patients, categorized into 100 patients without diabetes mellitus (Group 3A) and 100 patients with type 2 newly diagnosed diabetes mellitus (Group 3B). All the participants were investigated for Random blood sugar with capil lary blood samples to know whether any participants are newly diagnosed with Type 2 diabetes mellitus and they were further analyzed for fasting and postprandial blood sugar. The subgingival plaque samples were collected from the periodontal pockets and cu ltured in Sabouraud’s Dextrose Agar (SDA) plate and looked for the growth of Candida species. The samples that yielded Candida species were further analyzed for different types of candida species by standard microbiological procedures that include CHROM agar, direct microscopy, germ tube test, carbohydrate assimilation test and carbohydrate fermentation test. RESULTS: The overall prevalence of Candida species among 500 participants was 166 (33.2%). The most frequently isolated species in this study was C.albicans (23.6%) followed by C.glabrata (6%), C.tropicalis (3.2%) and C.krusei (0.4%). The overall prevalence of Candida species in this study was 48.5% in type 2 newly diagnosed diabetes mellitus patients (97 out of 200) and 23% in patients without diabetes mellitus (69 out of 300). The C.albicans was the predominant species in type 2 newly diagnosed diabetes mellitus patients. CONCLUSION: In this study the overall prevalence of Candida species was higher in stage II and stage III periodontitis patients with type 2 newly diagnosed diabetes mellitus. There was a greater predilection of Candida species in type 2 newly diagnosed diabetes mellitus patients with stage III periodontitis.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 241713701 |
| Uncontrolled Keywords: | Stage II periodontitis, stage III periodontitis, Type 2 diabetes mellitus, Candida species, Dental plaque or biofilm. |
| Subjects: | DENTAL > Periodontology |
| Depositing User: | Subramani R |
| Date Deposited: | 21 Feb 2021 17:01 |
| Last Modified: | 21 Feb 2021 17:01 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14371 |
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