Ragha Malika, S (2020) Microbial Analysis of Red Complex Organisms of the Whole Saliva in patients with Gingivitis and Gingival Recession using Next Generation Sequencing. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: The red complex bacteria which includes Porphyromonas gingivalis, and Tannerella forsythia, Treponema denticola are described as climax colonizers and are thought to play an important role in progression of periodontal disease. These bacterial species are usually found abundant in periodontal pockets, suggesting that they are associated with the dysbiosis of the periodontal environment. Technological developments in sequencing and identifying DNA and powerful bioinformatics tools have helped in characterization of microbiota of interest in relation to the total microbial load. AIM OF THE STUDY: The aim of this study was to evaluate the presence of the red complex bacteria in saliva using Next Generation Sequencing Technology in gingival health, gingivitis and gingival recession. MATERIALS AND METHODS: A total of 30 individuals seeking dental treatment in Ragas Dental College and Hospitals, Chennai, were involved in the present study, of which 10 were periodontally healthy individuals (control group), 10 were patients with gingivitis (test group 1) and 10 were patients with gingival recession (Test group 2). The salivary red complex was investigated with NGS technology using Illumina MiSeq sequencing method. Amplicons from V3-V4 hypervariable regions of 16S rRNA gene were sequenced. RESULTS: The results of the current study suggest that the Red complex bacteria were statistically significantly higher levels in gingivitis and gingival recession groups [Porphyromonas gingivalis P<0.001, Treponema denticola P<0.001 and Tanerella forsythia P<0.001] when compared to healthy subjects, but there is no significant difference between the test groups [Porphyromonas gingivalis P=0.85, Treponema denticola P=0.70 and Tannerella forsythia present in all 20 samples]. Therefore salivary red complex bacteria may be used as potential microbial risk markers for periodontal diseases. However, a longitudinal study with a greater sample size will be required to confirm the findings of the current study. CONCLUSION: Salivary red complex bacteria may be used a suitable candidate for the risk markers of gingivitis and gingival recession.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 241713153 |
| Uncontrolled Keywords: | Illumina MiSeq Sequencing, Next Generation Sequencing, 16S rRNA, Gingivitis, Gingival recession, salivary microbiome, Red complex organisms. |
| Subjects: | DENTAL > Periodontology |
| Depositing User: | Subramani R |
| Date Deposited: | 21 Feb 2021 15:25 |
| Last Modified: | 21 Feb 2021 15:25 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14355 |
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