Sreelakshmi, P (2020) Evaluation of relative efficacy of simvastatin in combination with hydroxyapatite in the treatment of human periodontal infrabony defects: A Six Months Randomized Controlled Clinical study. Masters thesis, J.K.K. Nattraja Dental College and Hospital, Komarapalyam.
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Abstract
BACKGROUND: Simvastatin has been found to cause increased bone formation in vitro and in animal studies. However, its effect on periodontal reconstruction in humans is yet to be determined. With this study an attempt was made to evaluate the effects of combination of simvastatin drug with Hydroxyapatite and also to compare the efficacy of this combination with that of Hydroxyapatite alone in the treatment of human periodontal infrabony defects. AIM AND OBJECTIVES: To perform comparative evaluation of efficacy of simvastatin in combination with hydroxyapatite in the treatment of infrabony defects in patients with chronic periodontitis. 1. In vitro assay was done to evaluate the osteogenic potential of simvastatin by assessing the bone markers RUNX2 and osteopontin in MG-63 osteoblast cell lines after incorporation with simvastatin. 2. To compare changes in probing pocket depth and clinical attachment level following therapy of both the groups. 3. To compare and measure the amount of bone fill in both the groups following therapy. MATERIALS AND METHODS: The in vitro study was conducted to evaluate the osteogenic potential of simvastatin by assessing the bone markers RUNX2 and osteopontin mRNA expression in MG-63 cell lines after incorporation with simvastatin using Real time PCR technique. The in vivo study was designed as a single blinded randomized controlled split mouth clinical trial for a period of 6 months. The study population comprised of 10 subjects and a total of 20 infrabony defects were treated. Group 1 consist of 10 sites, in which hydroxyapatite was placed (Control site) and Group 2 consist of 10 sites, in which simvastatin combined with hydroxyapatite was placed (Test site). Clinical parameters such as Plaque index (PI), Gingival index (GI), Oral hygiene index- Simplified (OHI-S), Probing Pocket Depth (PPD), Clinical Attachment Level (CAL) Infra Bony Defect depth (IBD) were evaluated. RESULTS: The results obtained were analyzed statistically and comparisons were made within each group using Students paired ‘t’ test. ‘p’ value between baseline, 3 months and 6 months post-operative were evaluated. The results of present study indicates simvastatin has stimulatory effects on the expression of the osteoblast specific genes RUNX2 and Osteopontin in MG-63 cell cultures. Both Test and Control group yielded favorable clinical results in periodontal infrabony defects. Both the groups exhibited significant amount of bone fill than the pre-operative levels and the mean bone fill was higher in simvastatin treated group. Infra Bony Depth reduction was greater in Test group with a statistically significant p – value of < 0.05. CONCLUSION: Simvastatin combined with hydroxyapatite showed a potential role in periodontal regeneration. As simvastatin can fulfill the dual action by inhibiting bone resorption as well as enhancing bone formation, it promises to be a viable alternative for periodontal regeneration in a cost-effective manner.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 241713101 |
| Uncontrolled Keywords: | RUNX2, Osteopontin, MG-63, Infrabony defects, Simvastatin, Hydroxyapatite, Clinical attachment level. |
| Subjects: | DENTAL > Periodontology |
| Depositing User: | Subramani R |
| Date Deposited: | 21 Feb 2021 14:52 |
| Last Modified: | 21 Feb 2021 14:52 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14352 |
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