Light Microscopy study of Microvasculature of Nonpregnant Fallopian Tube

Rubina Rachel Chandy, (2020) Light Microscopy study of Microvasculature of Nonpregnant Fallopian Tube. Masters thesis, Christian Medical College, Vellore.

[img]
Preview
Text
202301120rubina_rachel_chandy.pdf

Download (1MB) | Preview

Abstract

BACKGROUND: The microvasculature of the Fallopian tube includes the arterioles, capillaries, postcapillary venules (HEVs) and venules. The microvasculature architecture of Fallopian tube plays a vital role in the transport of ovum within the tube and the zygote to uterine cavity. The female genital tract is also part of mucosal associated lymphoid tissue and has intraepithelial lymphocytes, mainly T-lymphocytes. AIM OF THE STUDY: To study the microvasculature of nonpregnant Fallopian tube under light microscopy. OBJECTIVES: 1. Quantification of mucosal arterioles, capillaries, post-capillary venules (HEVs) and venules in non-pregnant Fallopian tubes under light microscopy. 2. To count and compare the number of CD4+ cells and CD8+ cells per unit length of epithelium in nonpregnant Fallopian tubes. 3. To count and compare the number of CD4+ cells and CD8+ cells per unit length of epithelium in postpartum Fallopian tubes. 4. To look for correlation between CD4/CD8 cells with the number of microvessels- arterioles, capillaries, venules and HEVs. MATERIALS AND METHODS Ethical approval was obtained from the institutional review board. Informed consent from 15 patients who underwent LSCS with sterilization after LSCS and 15 patients who underwent interval sterilization three months after delivery from the department of Obstetrics and Gynaecology, CMC Vellore was obtained. The ampullary part of both Fallopian tubes were obtained. The age of the patients ranged from 21 – 30 years. One half of the nonpregnant Fallopian tube tissue obtained was processed for immunostaining in the department of Anatomy. The other half was embedded in resin. Postpartum Fallopian tube specimens was processed for immunostaining. For immunostaining, CD4 and CD8 immunomarkers was used. The number of CD4+ve and CD8+ve cells was counted per unit length of epithelium using Cellsens software. Semithin sections will be taken from the resin blocks and stained with toluidine blue. The number of mucosal arterioles, capillaries, venules was counted per unit area. The data was analysed. Data was entered in epidata and statistical analysis was performed using SPSS version 24. The descriptive statistics, namely, the minimum, maximum, mean, standard deviation, median and inter-quartile intervals were obtained for the variables counted and measured. Mann-Whitney U test was done to compare the values of CD4 and CD8 positive cells in postpartum and nonpregnant Fallopian tube. Wilcoxon Signed Ranks test was done for comparison of CD4 and CD8 in postpartum and nonpregnant Fallopian tube. Simple linear regression was done to find out the correlation between microvessels and CD4 /CD8 IELs RESULTS: Of the microvessels, capillaries were more in number, followed by venules, HEVs and arterioles The number of CD8 positive IELs were significantly high in postpartum Fallopian than in nonpregnant Fallopian tube. Wilcoxon Signed Ranks test for comparison of CD4 and CD8 cells in post-partum Fallopian tube was highly significant. CONCLUSIONS: 1. The epithelium of non-pregnant Fallopian tube was made up of both simple columnar and stratified columnar epithelium. 2. Of the microvessels, the capillaries were more in number, followed by venules, HEVs and arterioles. 3. The mean number of arterioles/mm2 of mucosa was 11.87 with a median of 6 and inter quartile range of 15. 4. The mean number of capillaries/mm2 of the mucosa was 118.4 with median of 96 and inter quartile range of 121. 5. The mean number of venules/mm2 area of the mucosa was 21.6 with median of 12 and inter quartile range of 28. 6. The mean number of HEVs/mm2 mucosa was 16.93 with median of 8 and inter quartile range of 14. 7. The IELs were present in different levels of epithelium either singly or in rows. 8. The mean number of CD4 positive IELS per millimetre length of mucosa of postpartum Fallopian tube was 14.06±12.12 and of nonpregnant Fallopian tube was 27.33±33.34. 9. The mean number of CD8 positive IELS per millimetre length of mucosa of postpartum Fallopian tube is 94.33±27.9 and in non-pregnant Fallopian tube was 214.46±120.34. 9. Association of neutrophil with HEVs were seen. 10. The number of CD8 positive IELs were significantly high in nonpregnant Fallopian than in postpartum Fallopian tube. 11. There was no correlation between the microvessels and the number of CD4/CD8 positive IELs.

Item Type: Thesis (Masters)
Additional Information: 201733203
Uncontrolled Keywords: Light Microscopy study, Microvasculature, Nonpregnant Fallopian Tube, IELS – Intraepithelial Lymphocytes.
Subjects: MEDICAL > Anatomy
Depositing User: Subramani R
Date Deposited: 06 Feb 2021 04:14
Last Modified: 06 Feb 2021 04:14
URI: http://repository-tnmgrmu.ac.in/id/eprint/13918

Actions (login required)

View Item View Item