Vasanthakumar, J (2020) Comparative study of HIV, HCV and HBV screening by Third and Fourth Generation Elisa in Donated Blood. Masters thesis, The Tamilnadu Dr.M.G.R. Medical University, Chennai.
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Abstract
BACKGROUND: Transfusion-transmissible infections such as HIV, HCV and HBV are among the greatest threats to blood safety and potential serious chronic sequelae associated with readily transmitted agents. The implementation of these new highly sensitive 4th generation ELISA tests has significantly enhances early detection of HIV, HCV and HBV infection compare with 3rd generation ELISA. AIM OF THE STUDY: This study is aimed to compare the 4th generation ELISA with third generation ELISA for early detection of HIV, HCV and HBV infection among voluntary blood donors. MATERIALS AND METHODS: This Cross-sectional study was conducted over one year period from August 2018- July 2019 in the Department of Transfusion Medicine, The Tamil Nadu Dr.M.G.R. Medical University, Guindy, Chennai. All blood units were tested for HIV I&II, HCV antibodies and HBsAg using 3rd generation ELISA (Merilisa hiv 1 & 2 gen 3, Merilisa HCV and Merlisa HBsAg kits manufactured by Meril diagnostics). In addition donor units were screening with 4th generation HIV Ag-Ab, HCV Ag-Ab and HBsAg ELISA (Genscreen ULTRA HIV Ag-Ab kits, Monolisa HCV Ag-Ab ULTRA V2 kit and Monolisa HBsAg ULTRA ELISA kits manufactured by Bio Rad). RESULTS: Among total 373 voluntary blood donor samples, 3 were found to be seroreactive for HBsAg by 3rd and 4th generation ELISA. Only one sample was found to be seroreactive for HCV by 4th generation ELISA, this was found to nonreactive by 3rd generation ELISA. None of the samples were reactive for HIV by both 3rd and 4th generation ELISA. CONCLUSION: In the present study, there is no difference in detection of HBsAg and HIV between 3rd and 4th generation ELISA. In detection of HCV by 4th generation ELISA is found to be more sensitive than 3rd generation ELISA.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 201731004 |
| Uncontrolled Keywords: | HIV, HCV and HBV screening, Third and Fourth Generation Elisa, Donated Blood, Comparative study. |
| Subjects: | MEDICAL > Immunohaematology Blood Transfusion |
| Depositing User: | Subramani R |
| Date Deposited: | 05 Feb 2021 16:38 |
| Last Modified: | 05 Feb 2021 16:38 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/13901 |
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