Sivagami, P (2020) Neurobehavioral Evaluation of Ethanolic Flower Extract of Lawsonia Inermis in Mice. Masters thesis, Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Kanchipuram.
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Abstract
INTRODUCTION: Physiological organization of human body is determined by the central nervous system. Our unique human function, consciousness is characterized by CNS. Neurobehavioral disorders can result directly from disruption in brain function or complex interactions between brain and bodily atmosphere. Approximately 1.5 billion individuals are ailing from neurobehavioral illnesses worldwide and indeed the percentage share of global problem of disease due to neurologic dysfunction is predicted to rise up to 14.7% by 2020. Lawsonia inermis, popularly known as henna, has shown beneficial therapeutic effects such as antioxidant, anti-inflammatory, anticancer, antimicrobial, nootropic, antifertility, hepatoprotective and immunomodulatory effects but to our best knowledge, no scientific study has been conducted onits neurobehavioral activity. The aim of the study was to evaluate the neurobehavioral effect of ethanolic flower extract of Lawsonia inermis in experimental animals. MATERIALS AND METHODS: Neurobehavioral effects of EFELI were studied by oral administration of 200mg/kg and 400 mg/kg of EFELI in Swiss albino mice. Animals were divided into seven groups of six animals in each. Group 1: Animals divided into group A and B of 3 animals each for acute toxicity study. Group 2: Vehicle control, and given 0.1% sodium carboxy methyl cellulose p.o. Group 3: Reference control for antinociceptive activity and received Inj morphine 5 mg/kg i.p. Group 4: Reference control for Anxiolytic, locomotor and muscle relaxant activity, received Inj. diazepam 1 mg/kg i.p Group 5: Treated with EFELI 200mg/kg p.o. Group 6: Treated with EFELI 400mg/kg p.o. Group 7: Reserved for replacement in case of death The test drugs were directed 30 minutes prior to the commencement of study. For acute toxicity study,each animal in group A received EFELI in doses 200, 400 as well as 800 mg/kg p.o correspondingly and each animal in group B received EFELI in doses 1200, 1600 and 2000 mg/kg per oral correspondingly then noticed for 7 -10 days. Based on the data, LD50 of EFELI was determined as well as 1/10thand 1/5th of lethal dose selected for neurobehavioral learning. Anxiolytic activity was evaluated by means of Elevated plus maze and Hole board apparatus with Inj Diazepam as reference control. Locomotor and muscle relaxant activity were assessed by using Actophotometer and Rota rod respectively with Inj.Diazepam as reference control. Antinociceptive activity was assessed by using Eddy’s hot plate with Inj .morphine as reference control. RESULTS: The EFELI showed mild sedation at 2000mg/kg and that was considered as lethal dose.1/10th [200 mg/kg] and 1/5th [400mg/kg] of doses were used for the study. The animals treated with EFELI at 200mg/kg, showed significant increase (P<0.001) in the number of entries and the time spent in open arm (P<0.001) as well as decrease in (P<0.05) the number of entries and the time spentin closed arm (P<0.05) compared to control. At 400mg/kg significantly increase (P<0.001) the number of entries and time spent in open arm (P<0.001) and decrease in (P<0.01) the number of entries and the time spent in closed arm (P<0.01) compared to control. EFELI in doses 200mg/kg and 400mg/kg significantly increased the number of head dips (P<0.01) and (P<0.001) respectively as compared to control. EFELI (200mg/kg) showed less significant (P<0.01) decrease in locomotor activity. EFELI (400mg/kg) showed greater significant (P<0.001) decrease in locomotor activity which was comparable with control Diazepam. EFELI in doses 200mg and 400mg/kg showed significant (P<0.001) reduction in fall of time from rotating rods to 30.33±1.05 and 21.83±0.70 seconds thus showing dose dependent muscle relaxation effect. EFELI at 200 mg/kg showed less significant (P<0.01) decrease in the basal reaction time of 7.33±0.33 and the percentage protection was 34.11%. EFELI at 400mg/kg showed marked significant (P<0.01) increase in the basal reaction time of 9.17±0.31 and the percentage protection was 47.33%. CONCLUSION: It was concluded that, EFELI showed significant, anxiolytic, locomotor, muscle relaxant as well as antinociceptive effect in dose dependent manner. More extensive study is necessary to determine the exact mechanism of Lawsonia inermis flower extract by isolating the active principle, which leads to a safe herbal based promising therapy intended for neurobehavioral disorders.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 201716451 |
| Uncontrolled Keywords: | Ethanolic flower extract, Lawsonia inermis, neurobehavioral evaluation. |
| Subjects: | MEDICAL > Pharmacology |
| Depositing User: | Subramani R |
| Date Deposited: | 31 Jan 2021 14:30 |
| Last Modified: | 31 Jan 2021 14:30 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/13531 |
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