Effect of Cleistanthin A on vascular tension in isolated goat artery

Srisangeetha, G (2020) Effect of Cleistanthin A on vascular tension in isolated goat artery. Masters thesis, Christian Medical College, Vellore.


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BACKGROUND: Suicidal poisoning with Cleistanthus collinus was most common in rural south India. The consumption of leaves was either in the form of boiled decoction (fresh leaves boiled) or fresh leaves blended and filtered. Death rate was about 37.5% and was stated to be very high with the boiled decoction. Death occurs due to vasoplegic shock. There was no promising antidote to date. In this study, we isolated one of the two toxic compounds Cleistanthin A from aqueous extract of dried leaves of Oduvan. The effect of Cleistanthin A on vascular tension in the isolated goat artery was then studied. AIM OF THE STUDY: To observe the effect of Cleistanthin A isolated by preparatory HPLC on vascular tension in circular and longitudinal strips of small arteries from goat legs. METHOD: Shade dried leaves of C.collinus were soaked for 24hrs and boiled for 15 min. After cooling, this extract (DLRTB) was subjected to liquid-liquid chromatography by adding chloroform and pure Cleistanthin-A was isolated by Preparatory HPLC. An isolated artery from fresh goat legs was made into two different preparations: longitudinal strips and transverse cylinder. One end was tied to an organ bath with ECF solution, gassed with carbogen at 37ºC. The other end was connected to a force transducer and change in tension was recorded on adding Cleistanthin A. RESULT: Cleistanthin A did not cause any contractile or relaxant response in both circular and longitudinal smooth muscle at low molar (100μM) concentrations. In high molar (500μM) concentrations of Cleistanthin A causes a contractile response in circular smooth muscle (transverse cylinder) and did not cause any contractile or relaxant response in longitudinal smooth muscle. CONCLUSION: Cleistanthin A, one of the toxic principles of Cleistanthus collinus, did not exert any change in vascular tension of the longitudinal smooth muscle. But increases vascular tension in the circular smooth muscle at high molar(500μM) concentrations.

Item Type: Thesis (Masters)
Additional Information: 201715354
Uncontrolled Keywords: Cleistanthin A, HPLC, small artery, vascular tension.
Subjects: MEDICAL > Physiology
Depositing User: Subramani R
Date Deposited: 30 Jan 2021 17:19
Last Modified: 01 Mar 2021 02:27
URI: http://repository-tnmgrmu.ac.in/id/eprint/13504

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