Amritha, R S (2020) Effects of Smoking on Serum Total Antioxidant Capacity. Masters thesis, Thanjavur Medical College, Thanjavur.
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Abstract
INTRODUCTION: Cigarette smoking is one of the most important cause of early and preventable death and a significant public health concern throughout the globe. It has so many serious effects on human health such as chronic obstructive pulmonary disease, atherosclerosis and cancer. Cigarette smoke is highly complex aerosol and composed of 7,357 chemical compounds of different classes. Two main phases have been identified in cigarette smoke: tar phase and gas phase. Both phases are rich in oxygen, carbon, nitrogen and free radicals. Analysis has shown that a puff of cigarette contains about 1014 free radicals in the tar phase and 1015 free radicals in the gas phase. When we smoke cigarettes, many chemicals in the tar and gas phase enter in our body through our lungs and reach the tissues. Imbalance between antioxidants and oxidants formed in the body is a condition called oxidative stress. Examples of oxidants are Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS). Smoking produces a large amount of reactive oxygen species which has an influence on normal cellular function and causes changes in the inflammatory markers. Many researchers have found that there is a direct relationship between oxidative stress and pathological diseases. AIM OF THE STUDY: The Aim of the study was to evaluate the effects of smoking on Serum Total Antioxidant Capacity in smokers and to compare the levels of TAC between adult male smokers and non-smokers. METHODS: This study was a case control study that included 80 subjects of which 40 were non-smokers and 40 were smokers in the age group of 30-50 years. The non- smokers were in control group and smokers in cases group. All the participants were non-athletes, non-alcoholics and had not participated in regular exercise / diet programs for the period preceding 6 months. Smoking history of at least 10 cigarettes a day for 5 years was considered as inclusion criteria in smokers group. Patient with Type II Diabetes mellitus, Respiratory diseases, Cardio-vascular diseases, Cancer, Kidney dysfunction and other chronic diseases were excluded in this study. Blood samples were collected from 8-9 AM after fasting 12 hours overnight. Serum TAC was measured by FRAP spectrophotometric assay. RESULTS: The results were considered statistically significant if the p value was < 0.05. In our study, serum TAC was evaluated in smokers and non-smokers. The study findings showed that serum TAC levels were lower in smokers when compared to non-smokers. A negative correlation was found between Serum TAC and duration of smoking. CONCLUSION: Our study revealed decreased TAC levels in smokers which may be helpful in predicting oxidative stress related disease earlier and can reduce the progression of the disease. Hence we conclude that quitting smoking, exercise, good nutrition and use of antioxidants supplementation can improve the TAC levels in smokers.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 201715201 |
| Uncontrolled Keywords: | Smoking, Cigarette, Oxidative stress, Total Antioxidant Capacity. |
| Subjects: | MEDICAL > Physiology |
| Depositing User: | Subramani R |
| Date Deposited: | 30 Jan 2021 16:52 |
| Last Modified: | 01 Mar 2021 03:10 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/13490 |
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