Determining the Effect of Intensity of Host Tumour NK Cell & Cytotoxic T Lymphocyte Infiltration on Tumor related Parameters of Aggressiveness in Patient with Mutant P53 Expressing Colorectal Carcinoma

Rajalakshmi, S (2020) Determining the Effect of Intensity of Host Tumour NK Cell & Cytotoxic T Lymphocyte Infiltration on Tumor related Parameters of Aggressiveness in Patient with Mutant P53 Expressing Colorectal Carcinoma. Masters thesis, Tirunelveli Medical College, Tirunelveli.

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Abstract

INTRODUCTION: Colorectal Cancer (CRC) arises through various mechanisms and the frequent mechanism involves p53 mutation. Wild p53 communicates to the adaptive immune system and control the cytotoxic T lymphocyte response to cancer cells. Mutation in p53 impacts the immunoreactivity of cancer cells and results in impaired cytotoxic T lymphocyte response. Cytotoxic T lymphocyte and natural killer cell in tumor microenvironment might have immunoprotective effect on CRCs. AIM OF THE STUDY: To evaluate the relation between CD8 and CD56 expression and various clinico-pathologic parameters of tumor aggressiveness in p53 expressing CRCs. MATERIALS AND METHODS: 51 paraffin-embedded tissue blocks of colectomy specimens were used. Data like age, gender, tumour location, tumour size, histological type, tumour grade, pT stage, nodal metastasis, tumor margin, tumor budding, pericolic deposits, vascular and neural invasion were analysed. Four sections of 4μ thickness from paraffin embedded tissue block were taken. First section was taken for Haematoxylin and Eosin stain and other three for immunohistochemistry (p53, CD8, CD56 antibodies). RESULTS: p53 staining was positive in 75% (38/51) of the cases. p53 mutation was most common on left side CRCs and showed higher pT stage. CD8 expression correlated with nodal metastasis in p53 expressing CRCs (p=0.027). However, there was no significant association between CD8 protein expression and other clinico-pathologic variables in p53 expressing CRCs. CONCLUSION: p53 mutation was associated with higher pT stage. Enrichment of CD8+ T lymphocyte may limit the nodal spread in p53 positive colorectal carcinomas.

Item Type: Thesis (Masters)
Additional Information: 201613306
Uncontrolled Keywords: Colorectal carcinoma, p53, CD8, CD56, CD8+ T lymphocyte.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 29 Jan 2021 17:19
Last Modified: 29 Jan 2021 17:19
URI: http://repository-tnmgrmu.ac.in/id/eprint/13420

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