Comparison of Immunochromatography with RT-PCR for detection of Rotavirus in Fecal Samples.

Manjula, G (2013) Comparison of Immunochromatography with RT-PCR for detection of Rotavirus in Fecal Samples. Masters thesis, Tirunelveli Medical College,Tirunelveli.

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Abstract

INTRODUCTION : Diarrhoeal diseases have been recognized in humans since antiquity. Diarrhoeal diseases are one among the most common illnesses in infants and young children all over the world. Acute diarrhoeal diseases are the major cause of mortality and morbidity in children especially in developing countries like India. They are one among the six major causes of childhood mortality accounting for 18% of the deaths in children less than five years, with highest mortality in developing countries. Diarrhoeal diseases have been recognized in humans since antiquity. Diarrhoeal diseases are one among the most common illnesses in infants and young children all over the world. Acute diarrhoeal diseases are the major cause of mortality and morbidity in children especially in developing countries like India. They are one among the six major causes of childhood mortality accounting for 18% of the deaths in children less than five years, with highest mortality in developing countries. AIMS AND OBJECTIVES : 1. To find out the prevalence of rotavirus associated diarrhoea in children less than 2 years. 2. To evaluate the rapid immunochromatographic test in rotavirus antigen detection. 3. To compare the efficiency of rapid ICT against RT-PCR in detection of rotavirus infection. MATERIALS AND METHODS : The present study was conducted at the Department of Microbiology , Tirunelveli Medical College, Tirunelveli from May to November 2012 to detect the prevalence of rotavirus associated diarrhea in children less than 2 years of age and to evaluate the efficiency of rapid immunochromatographic test [ICT] in rotavirus antigen detection and also to compare its performance against Polymerase chain reaction [RT-PCR], the gold standard test. A total of 100 stool samples were collected from eligible children. Inclusion criteria: Children less than 2 years of age with loose stool without blood and mucus. Children requiring hospitalization. Exclusion criteria: Children more than 2 years of age with loose stool. Children less than 2 years of age with loose stool with blood and mucus. All 100 samples were tested for rotavirus by both rapid immunochromatography and RT-PCR. RESULTS : All the results obtained from the study were analysed stasticially for their completeness, consistency and accuracy by the parameters like mean, median and percentages. The differences of above parameters were tested by the parametric tests like ‘Z’ and‘t’ and non-parametric test like χ2 test, which was applicable wherever. The results of Rapid ICT and RTPCR were compared by Mcnemar’s χ2 test and confirmed by ‘Z’ test of proportions. The above statistical procedures were performed by IBM SPSS Statistics 20. The P- Values of less than 0.05 were considered as statistically significant in two tailed test (P<0.05). CONCLUSION : The present study revealed that rotavirus is the major cause of acute diarrhoea in infants and children less than two years of age. So, all children with acute gastroenteritis should have their stool examined for rota virus for purpose of correct diagnosis to avoid unnecessary use of antibiotics and emergence of drug resistance. Though ICT has lower sensitivity than PCR ,it can be used as a alternate test for rapid diagnosis in situations of emergency testing, as an outpatient procedure. Highly sensitive detection method like RT-PCR is necessary to find out the exact number of cases with rotavirus infection. Further molecular studies are essential to know the accurate information of rotavirus serotypes which will be helpful in formulating vaccines in future.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Immunochromatography ; RT-PCR ; Detection ; Rotavirus ; Fecal Samples ; Comparison.
Subjects: MEDICAL > Microbiology
Depositing User: Subramani R
Date Deposited: 14 Aug 2017 02:59
Last Modified: 14 Aug 2017 02:59
URI: http://repository-tnmgrmu.ac.in/id/eprint/1341

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