Role of Podoplanin as IHC Marker in Detection of Lymphovascular Invasion in Colorectal Carcinomas: An Early Predictor for Lymph Node Metastasis

Gugan, C (2020) Role of Podoplanin as IHC Marker in Detection of Lymphovascular Invasion in Colorectal Carcinomas: An Early Predictor for Lymph Node Metastasis. Masters thesis, Coimbatore Medical College, Coimbatore.

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Abstract

INTRODUCTION: This is a prospective study which included 32 surgically resected specimens for Colorectal carcinomas irrespective of their clinical staging. The study was conducted in the Department of Pathology, Coimbatore Medical College Hospital. AIM OF THE STUDY: Aim of this study is to determine the role of immunohistochemical marker-Podoplanin (D2-40) in identification of Lymphatic invasion and to correlate with clinicopathological features. OBJECTIVES: 1. Detection of lymphatic invasion in colorectal cancers using H&E staining and by IHC marker- Podoplanin. 2. To correlate with histopathological grading and other clinicopathological features. DISCUSSION: Review of literatures shows the importance of identification of Lymphovascular invasion in deciding the prognosis and management for these patients. In H&E stained sections there can be interobserver variations in identifying lymphatic invasion, as the artifactual spaces can mimic lymphatic vessels. The specimens were grossed and routine Hematoxylin and eosin staining was done. Microscopic examination of the slides was done and the findings are noted. Appropriate blocks were selected for immunohistochemistry using Podoplanin as primary antibody and microscopic examination of the slides was done to identify lymphatic invasion. The correlation of lymphatic invasion with clinicopathological features like age, gender, presenting symptom, site of tumor, extent of tumor, depth of tumor, histological type, histological grade, lymph node status and TNM staging are studied. The comparison of H&E staining with IHC marker Podoplanin in identification of lymphatic invasion shows 100% sensitivity of podoplanin for lymphatic endothelium, thereby areas mimicking lymphatic vessels can be identified without interobserver variations.

Item Type: Thesis (Masters)
Additional Information: 201713252
Uncontrolled Keywords: Podoplanin, IHC Marker,Detection of Lymphovascular Invasion, Colorectal Carcinomas, Early Predictor, Lymph Node Metastasis.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 29 Jan 2021 16:34
Last Modified: 29 Jan 2021 16:34
URI: http://repository-tnmgrmu.ac.in/id/eprint/13408

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