Raj Babitha, A (2016) A comparative study of normal saline and glycerol rehydration techniques of air dried smears as an alternative for wet cervicovaginal smears. Masters thesis, Sree Mookambika Institute of Medical Sciences, Kulasekharam.
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Abstract
Cervical cancer is one of the most common cancers in women which leads to 2,70,000 deaths worldwide. Early detection of cervical cancer by Pap smear. Improper fixation of PAP smear can lead to artifacts which may even render the specimen uninterruptable or unsatisfactory. To overcome this obstacles air dried and unfixed slides are rehydrated by normal saline and aqueous glycerin resulting in staining which is comparable or superior to conventionally wet fixed Pap stained smears. Drying of cervical smears for two hours followed by rehydration with 50% glycerol then staining by routine Pap stain is found out to be superior to wet fixed smears. Rehydration of dry cervical smears with 0.85% normal saline followed by routine Pap staining is found to be comparable to wet fixation technique. Both the techniques are simple and reasonable for routine screening of cervical cancers especially in mass screening programs and resource limited settings.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Papanicolaou Stain ; Carcinoma of Cervix ; Transformation Zone ; Air Drying Artifacts ; 95% Ethyl Alcohol ; The Bethesda System. |
| Subjects: | MEDICAL > Pathology |
| Depositing User: | Punitha K |
| Date Deposited: | 10 Jul 2017 11:18 |
| Last Modified: | 26 Nov 2018 17:33 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/1314 |
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