A Comparative Analysis of Antibiotic Resistance Pattern of Metallo-Β-Lactamase And Non-Metallo-Β-Lactamase Strains of Pseudomonas aeruginosa Isolated From Chronic Suppurative Otitis Media

Minu George, (2017) A Comparative Analysis of Antibiotic Resistance Pattern of Metallo-Β-Lactamase And Non-Metallo-Β-Lactamase Strains of Pseudomonas aeruginosa Isolated From Chronic Suppurative Otitis Media. Masters thesis, Madurai Medical College, Madurai.

[img]
Preview
Text
200400317minu_george.pdf

Download (2MB) | Preview

Abstract

BACKGROUND: Chronic suppurative otitis media (CSOM) is a notorious infection and a major health problem in developing countries causing serious local damage and threatening complications. Early and effective treatment based on the knowledge of causative micro organisms and their antimicrobial sensitivity ensures prompt clinical recovery and possible complications can thus be avoided. AIM OF THE STUDY: The aim of this study was to isolate the organisms associated with CSOM, to detect the antibiogram of the aerobic isolates, to study the prevalence of Metallo-beta-lactamase producing Pseudomonas aeruginosa and to compare the antibiotic resistance pattern of MBL producing and non-MBL producing Pseudomonas aeruginosa. MATERIALS AND METHODS: A total of 150 patients clinically diagnosed of CSOM were enrolled in the study and the samples were collected from the ENT department of Government Rajaji Hospital Madurai, from January 2015-june 2016 .The samples obtained from each patient using sterile cotton swabs and cultured for microbial flora. Drug susceptibility testing for aerobic isolates was conducted using Kirby Bauer disc diffusion method. Screening for MBL was done by Imipenem (10μg) disk and phenotypic confirmation of MBL was done by combined disk method. Genotypically confirmed by PCR. (blaVIM). RESULTS: The most common causative organisms isolated were Pseudomonas aeruginosa (56.44%), followed by Staphylococcus aureus(25.77%) amongst the 163 aerobic isolates The Metallo-beta-lactamase production among the Carbapnem resistant isolates were 6 (60 %) The percentage of Metallo-beta–lactamase production among Pseudomona aeruginosa was 6.52%. High level of drug resistance was observed for Gentamicin and Amikacin 83.33% TheMetallo-Beta-lactamase producing Pseudomonas aeruginosa were shown to exhibit more resistance than non metallo-beta-lactamase producing isolates. CONCLUSION: Knowing the etiological agents of CSOM and their antimicrobial susceptibility is of essential importance for an efficient treatment, prevention of both complications and development of antibiotic resistance and finally, the reduction of the treatment costs. Judicious use of antibiotics, rapid isolation of patients suspected to have Carbapenm resistant Pseudomonas aeruginosa infections and regular testing of all isolates for Metallo-beta-lactamase production among Pseudomonas aeruginosa is recommended for the prevention of tramission of Carbapenem resistant Pseudomonas aeruginosa.

Item Type: Thesis (Masters)
Additional Information: Reg.No.201414151
Uncontrolled Keywords: Chronic Suppurative Otitis Media, Pseudomonas aeruginosa, Metallo-beta–lactamases.
Subjects: MEDICAL > Microbiology
Depositing User: Subramani R
Date Deposited: 18 Jul 2020 17:15
Last Modified: 19 Jul 2020 03:00
URI: http://repository-tnmgrmu.ac.in/id/eprint/12542

Actions (login required)

View Item View Item