A Study on Comparison of different Phenotypic methods for detection of Extended Spectrum Beta Lactamase Production among Enterobacteriaceae in Urinary Tract Infection in a Tertiary Care Centre

Sasirekha, R (2017) A Study on Comparison of different Phenotypic methods for detection of Extended Spectrum Beta Lactamase Production among Enterobacteriaceae in Urinary Tract Infection in a Tertiary Care Centre. Masters thesis, Madurai Medical College, Madurai.

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Abstract

INTRODUCTION: Urinary Tract Infections are serious threat to human health affecting millions of people every year resulting in high morbidity and mortality. Escherichia coli, Klebsiella spp, Proteus mirabilis of Enterobacteriaceae are the commonest uropathogens. Antibiotic resistance in uropathogens is increasing worldwide most of the uropathogens of Enterobacteriaceae carry resistance gene for Extended Spectrum Beta Lactamase production on plasmid that can easily spread from organism to organism. Aims and OBJECTIVES: To identify and to compare the rate of detection of ESBL strains by different phenotypic methods and confirmation by genotypic method among Enterobacteriaceae of UTI. MATERIALS AND METHODS: About 122 isolates of Enterobacteriaceae isolated from 400 urine samples of both inpatient and out patients of Government Rajaji Hospital, Madurai during Sep 2015-Aug 2016 were included. ESBL strains were identified by screening methods and confirmed by Phenotypic methods DDST,PCT, CHROM agar, Etest. The results are confirmed by presence of ESBL genes by genotypic method. RESULTS: Among 81 isolates of Escherichia coli 34(41.98%), among 32 isolates of Klebsiella spp 14(43.75%),among 6 isolates of Proteus mirabilis 2(33.33%) were ESBL producers. Totally in Enterobacteriaceae 20.49% by DDST method and 40.98% by PCT, ESBL CHROM agar and E-test were detected as ESBL producers. The commonest gene isolated was CTX-M either alone or in combination with other genes TEM and SHV. CONCLUSION: For early detection of ESBL producers PCT can be performed in Microbiology Laboratory as they are simple to perform and cost effective.

Item Type: Thesis (Masters)
Additional Information: Reg.No.201414102
Uncontrolled Keywords: UTI, ESBL, DDST, CHROM agar, PCT, E-test, Enterobacteriaceae.
Subjects: MEDICAL > Microbiology
Depositing User: Subramani R
Date Deposited: 18 Jul 2020 17:10
Last Modified: 19 Oct 2021 06:49
URI: http://repository-tnmgrmu.ac.in/id/eprint/12541

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