Vallabi, R (2017) Diagnostic Validity of Human Papilloma Virus E6 mRNA assay as a triage test for cervical cytology and HPV DNA test with biopsy as gold standard. Masters thesis, Madras Medical College, Chennai.
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Abstract
BACKGROUND: Cervical cancer is second most frequent cancer of women in the world HPV infection is now a well established cause of cervical cancer. Currently used screening programmes are detecting cases in which only 15-20 percent of women found to have an abnormality significant enough to need treatment, other 80-85 percent were probably false alarms. The oncogenic process in cervical cancer is initiated and mediated by upregulation of E6/E7 mRNA proteins and their over expression. So HPV E6 mRNA detection as a triage test could actually detect a neoplastic transformation and may help to reduce the false alarms. AIMS AND OBJECTIVES: To validate the expression of HPV E6 mRNA as a triage for cervical cytology and HPV DNA .Biopsy is used as gold standard for screening. MATERIALS AND METHODS: This a prospective study conducted in Institute of Social Obstetrics and Govt. Kasturba Gandhi hospital, Madras medical college, 41 cases positive for VIA/VILI in colposcopy were selected and subjected to conventional cytology and cervical biopsy, part of biopsy was collected in RNA later solution and were typed for HPV DNA 16& 18, HPV E6 mRNA Expression. The results of above test are compared with histopathology as gold standard. RESULTS: The specificity of E6 mRNA was 100% for both high grade and low grade lesions. But the sensitivity was less than HPV DNA and cytology for both low and high grade lesions. With increasing severity of lesion expression of E6 mRNA was found to increase. CONCLUSION: Low positivity rate and the high specificity makes E6 mRNA better test in a triage setting.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No.201413009 |
| Uncontrolled Keywords: | Cervical Cancer, human papilloma virus, E6Mrna. |
| Subjects: | MEDICAL > Pathology |
| Depositing User: | Subramani R |
| Date Deposited: | 18 Jul 2020 16:23 |
| Last Modified: | 18 Jul 2020 16:23 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/12530 |
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