Rajkohila, J (2014) Morphometric analysis of CD1a positive Langerhans cells in diseased human lung tissue by immunohistochemistry. Masters thesis, Christian Medical College, Vellore.
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Abstract
INTRODUCTION : The immune system is a complex defence system in the human body concerned with the state of being immune to the invasion by bacteria, viruses and other foreign bodies. It is made up of a network of cells, tissues and lymphoid organs. Protection against microbial infection is offered by two components of this system namely innate and acquired immunity. Innate (non adaptive/ natural immune response) is the inborn resistance present even before the first entry of a pathogen into the host. Acquired (adaptive immunity) is absent at the time of first exposure but resistance develops after being exposed to the pathogen. Innate immunity offers the first line of defence immediately after the microbial entry into the body. Normally when a microbe is not cleared by innate immune response the acquired immune response comes forward to defend the host. Innate and acquired immune responses co-operate and interact together to produce an effective clearance of the microbe. OBJECTIVES: To study the distribution and morphology of CD1a positive Langerhans cells (LCs) in human lung tissue in obstructive pulmonary diseases, benign and malignant diseases of the lung and quantify them. METHODS: After getting informed consent, human lung tissue samples were collected from 30 patients who underwent pulmonary surgery in Christian Medical College, Vellore and were processed for immunohistochemical staining with mouse monoclonal antihuman CD1a antibody. The number of CD1a positive LCs per square area was counted using CellSens Standard image analyzer software. Statistical analysis was done using SPSS version 17. RESULTS: CD1a positive LCs were predominantly distributed in the bronchiolar compartment and were absent in alveolar compartment. They were also present in the interstitium and BALT in bronchiectasis, carcinoid and chondroid hamartoma. Of the obstructive pulmonary diseases studied, bronchiectasis with aspergilloma had more number of CD1a positive LCs. In adenocarcinoma, their number correlated inversely with the tumour grade. The mean number of CD1a positive LCs was highest in pleural metastasis of metastatic thymoma. There was no significant difference in the mean number of CD1a positive LCs across smokers and non smokers. CONCLUSION: In this study CD1a positive LCs were present in 28 lung biopsies of 30. CD1a positive LCs were predominantly distributed in the bronchiolar compartment in all the conditions studied. • Alveolar compartment was negative for CD1a positive LCs. Few CD1a cells were present in the interstitium in bronchiectasis, carcinoid and chondroid hamartoma and also in BALT in bronchiectasis, bronchiectasis with interstitial pneumonia and chondroid hamartoma. • Atypical CD1a positive cells were found in alveoli in few cases of bronchiectasis, bronchiectasis with interstitial pneumonia, congenital cystic adenomatoid malformation and carcinoid tumour. • Of the obstructive pulmonary diseases studied, bronchiectasis with aspergilloma had more number of CD1a positive LCs. • This is probably, the first immunohistochemical study that has reported the presence of CD1a positive LCs in the bronchiolar epithelium in the case of congenital cystic adenomatoid malformation, chondroid hamartoma and in pleural metastasis of metastatic thymoma. • Of the primary malignant disease studied, the number of CD1a positive LCs were very few in adenocarcinoma and this correlated inversely with the tumour grade. • In pleural metastasis of metastatic thymoma huge number of CD1a positive LCs were seen in bronchiolar epithelium. • There was no evidence of significant difference on comparison of CD1a positive LCs across different disease conditions. and also on comparison of CD1a positive LCs across smokers and non smokers. • There was no evidence of significant difference on comparison of CD1a positive LCs across male and female.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No.20117163 |
| Uncontrolled Keywords: | Langerhans cells, CD1a, bronchiectasis, chondroid hamartoma, metastatic thymoma. |
| Subjects: | MEDICAL > Anatomy |
| Depositing User: | Subramani R |
| Date Deposited: | 09 Jul 2020 11:09 |
| Last Modified: | 09 Jul 2020 11:16 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/12513 |
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