Pramod, S Prasad (2013) The effect of 5% Sodium hypochlorite, 17% Ethylenediaminetetraacetic acid and Triphala on two different rotary Nickel-Titanium instruments: An Atomic Force Microscopy and Energy Dispersive X-Ray Spectroscopy analysis. Masters thesis, Rajas Dental College and Hospital, Tirunelveli.
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Abstract
The aim of this in-vitro study was to evaluate the effect of sodium hypochlorite (NaOCl), ethylenediamine tetraacetic acid (EDTA) and triphala on surface characteristics of ProTaper and iRaCe rotary nickel-titanium instruments using Atomic Force Microscope (AFM) and Energy Dispersive X-Ray Diffraction Spectroscopy (EDS). A total of eight nickel-titanium rotary endodontic files, four files each of ProTaper – S2 (Dentsply, Switzerland) and iRaCe – R3 (FKG dentaire, Switzerland) were used. Three files each from ProTaper and iRaCe were immersed in 5.25% sodium hypochlorite, 17% ethylene diamine tetraacetic acid and triphala separately for five minutes. One file each from ProTaper and iRaCe were evaluated without immersion in irrigants. Nine perfect squares of 2x2, 5x5 and 10x10 micrometer were examined on a 3mm section taken from the middle portion of the files using Atomic Force Microscope (AFM). The Roughness average (Ra), Root Mean Square (RMS) and Mean Height of Roughness Profile Elements (Rc) of the scanned profiles were then recorded. The same samples were then evaluated using Energy Dispersive X-Ray Spectroscopy (EDS) to get the elemental characterization of the instrument surface. Data were analyzed by means of Student’s ‘t’ test, One Way ANOVA and Duncan’s Multiple Range Test. The three-dimensional AFM images of the surfaces of all the Protaper and iRaCe instruments including the new and those immersed in the three different irrigants showed topographic irregularities at the nanometric scale. Ra, RMS and Rc values of new Protaper files were significantly lower compared to iRaCe files (P < 0.05). Except for the EDTA immersed, the other three samples of Protaper files (new, sodium hypochlorite and triphala immersed files) showed no significant difference in their surface roughness. The NaOCl and EDTA immersed iRaCe files have shown a very highly significant increase in surface topography when compared to the new and triphala immersed iRaCe files (P < 0.001). The treated Protaper and iRaCe instruments image revealed an increase in the surface roughness compared to their respective controls. Among the immersed files, the maximum Ra value was observed for the NaOCl immersed iRaCe files and the minimum Ra value was observed for the triphala immersed Protaper files.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | 5% Sodium hypochlorite, 17% Ethylenediaminetetraacetic acid, Triphala, two different rotary Nickel-Titanium instruments, Atomic Force Microscopy, Energy Dispersive X-Ray Spectroscopy analysis. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Mar 2020 14:46 |
| Last Modified: | 24 Mar 2020 14:46 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/12462 |
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