An Exvivo Comparative study to Evaluate the Efficacy of Three Root Canal Irrigation Systems in Reduction of Enterococcus Faecalis

Diana Stanly, (2012) An Exvivo Comparative study to Evaluate the Efficacy of Three Root Canal Irrigation Systems in Reduction of Enterococcus Faecalis. Masters thesis, Ragas Dental College and Hospital, Chennai.

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Abstract

The purpose of this an ex-vivo study is to evaluate the efficacy of three irrigation techniques in reduction of E. faecalis in root canal. 55 intact maxillary anteriors were used in this study. Root length was standardized to 20mm. Teeth were mounted in self cure resin and autoclaved . Teeth were contaminated with E. faecalis and stored for 7 days. After contamination, teeth were randomly distributed into 3 experimental groups of 20 teeth each and control group of 10 each. Group I: irrigated with NaviTip FX; Group II: irrigated with Max-i-probe, Group III, irrigated with the Endovac system. Group IV (Control group) irrigated with 20 ml syringe and using 27 gauge needle. 3% NaOCl and17% (EDTA) were the irrigants used in all experimental groups and control group was irrigated with saline solution. 20ml syringe was used to deliver irrigant for all the groups. Root canal were instrumented by Protaper rotary instrument till size F5. After chemo-mechanical procedures bacterial samples taken from the root canal were cultured, and incubated for 2 days in Brain heart infusion agar and the colony-forming units (CFUs) were counted. All the experimental groups showed significant bacterial reduction when compared to group IV (control group). Among experimental groups, Group III (Endovac) showed less number of bacterial colonies. There was no statistical significance between the Group I (Navitip FX) and Group II (Max I probe). CONCLUSION: Under the limitations of the present study it can be concluded that: 1. All three newer irrigation delivery system (Navitip FX, Maxi-i-probe, Endovac) have been found to be effective in the reduction of E. faecalis. 2. Among the three irrigation systems Endovac showed maximum reduction of colony forming units. 3. Between Navitip FX and Max-i-probe delivery systems which were used under positive pressure, showed no statistical significance in reduction of E. faecalis population. 4. The results of this study confirmed that apical negative pressure technique has high potential to achieve better antimicrobial effect compared to the traditional irrigation delivery system.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Efficacy, Three Root Canal Irrigation Systems, Reduction of Enterococcus Faecalis.
Subjects: DENTAL > Conservative Dentistry and Endodontics
Depositing User: Subramani R
Date Deposited: 24 Mar 2020 05:50
Last Modified: 24 Mar 2020 05:50
URI: http://repository-tnmgrmu.ac.in/id/eprint/12452

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