Soundarya, S (2012) Analysis of Growth, Phenotypic characteristics and Expression of STRO1 and CD106, in Dental Pulp Stem Cells and Stem cells from Human Exfoliated Deciduous teeth: A Cell culture study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
In this study we successfully isolated and cultured five Dental Pulp Stem Cells (DPSC) from twelve samples of permanent teeth and six Stem cells from Human Exfoliated Deciduous teeth (SHED) from nine deciduous teeth. We compared the growth characteristics and the phenotype of both the cell populations. We also evaluated the immuno histochemical expression of STRO1 and CD 106 on these cells. • Estimation of growth curve and its derivatives revealed that the doubling time of DPSCs ranged from 37 hours to 144 hours. This long population doubling time indicate their loss of proliferation and entering the process of differentiation. SHED also had similar doubling time ranging from 13 hours to 246 hours. • The subpopulation analysis of both DPSCs and SHED revealed no statistically significant difference between the two groups. (p>0.05). • The seeding efficiency of DPSCs ranged from 37 to 112% but that of SHED ranged from 42.6 to 119.4. • Both DPSCs and SHED had higher proportion of F2 subpopulation, indicating slow proliferation. • The morphologically characterized DPSCs and SHED did not express the early mesenchymal markers, like STRO1 and VCAM. • Further study using a larger panel of antibodies is required to better localize and characterizethe stem cell niches of DPSCs and SHED apart from their perivascular locations. • Given the easy accessibility of pulp stem cell the molecular studies of DPSC and SHED with more number of samples should be done. • In future such characterization would improve the scope and utility of these cells in clinical dentistry and therapeutics.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Growth, Phenotypic characteristics, Expression, STRO1 and CD106, Dental Pulp Stem Cells, Stem cells,Human Exfoliated Deciduous teeth. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Mar 2020 04:48 |
| Last Modified: | 24 Mar 2020 04:48 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/12449 |
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