Kokila, P (2019) A Toxicity study on “Pattai Chooranam”. Masters thesis, Government Siddha Medical College, Palayamkottai.
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Abstract
The medicine PATTAI CHOORANAM was taken for the dissertation work based on Anuboga Vaidya Navaneetham, Part IX, 1975, Pg. no: 59. The aim of this dissertation was to study the acute and sub-acute toxicity effect of the medicine PATTAI CHOORANAM administered at various presumed several dosages in the experimental animals. The ingredients of PATTAI CHOORANAM were; Parangipattai, Nellikai Ganthagam Sathikai, Sathipathiri, Lavangam, Sirunagapoo, Amukkara Kizhangu, Kodiveli Verpattai, Sadamanjil, Pachai Karpooram. The samples were procured from Tirunelveli Town, Tirunelveli. The drug was analysed for its physicochemical properties and contents by using qualitative biochemical analysis and modern techniques such as ICP-OES, FTIR and XRD. Depending upon the results of this analysis the contents of test sample was identified. By Scanning Electron Microscope (SEM) the size of the particles about 0.5 - 5μ micron. The raw samples were taken for purification and the test medicine was prepared, as per method narrated in the literature. The study was conducted at Nandha College of Pharmacy, Erode. To evaluate the acute toxicity study 18 Wistar Albino Rats were selected and divided into 6 groups (Group I Control II, III, IV, V, VI) and they were administered with the drug with different graded doses ranging from Control Group (Distilled Water) 1ml/kg, 5mg/kg, 50mg/kg, 300mg/kg, 1000mg/kg and 2000mg/kg Body weight of animal orally. Daily the animals were observed for clinical signs and mortality. The drug did not produce any mortality and it is safe up to 2000mg/kg body weight. Sub-acute toxicity study was conducted for about 28 day duration. No signs of toxicity were observed in animals from different dose groups during the dosing period of 28 days. The blood samples were taken prior to sacrifice. The blood samples were sent to laboratory for haematological evaluation. No significant Haematological changes occurred in Group II (300mg/kg body weight) animals. But changes occurred in Group III (600mg/kg body weight) and Group IV (1200mg/kg body weight) animals. Both the control and the drug treated group showed constant weight gain from day 0 to day 28. No notable deviation in terms of body weight, food intake and water intake were observed in both the drug treated groups compared with the normal control. There were no remarkable histopathological changes in Group II (300mg/kg body weight) animals. But Mild changes occurred in liver (Male and Female) and heart (Female) in Group III and IV (600mg/kg body weight and 1200mg/kg body weight) animals. By Acute and Sub-acute toxicity studies, the drug PATTAI CHOORANAM is found to be safe up to 300mg/kg body weight. Further studies needed the dose above 300mg/kg body weight for the long period as sub chronic and Chronic toxicity studies. CONCLUSION: The PATTAI CHOORANAM was prepared using traditional literature sources and it was characterized by using various analytical techniques with PLIM guidelines. Finally it was assessed the safety of PATTAI CHOORANAM in acute and sub-acute toxicity studies through animal experiments. From the acute toxicity study it was observed that the administration of PATTAI CHOORANAM up to the dose of 2000mg/kg body weight of animal did not produced drug related toxicity and mortality. So No-Observed-Adverse-Effect- Level (NOAEL) of PATTAI CHOORANAM is 2000mg/kg body weight. In sub-acute toxicity study found Hematological and Histopathological changes in 600mg/kg and 1200mg/kg body weight (Group III and IV) of animals and in the dose 300mg/kg bodyweight (Group-II) of animals did not produced any Hematological and Histopathological changes. Therefore Pattai Chooranam can be significantly safe up to the dose 300mg/kg body weight in oral administration for a long term.
| Item Type: | Thesis (Masters) |
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| Additional Information: | (Reg.No. 321616004) |
| Uncontrolled Keywords: | Pattai Chooranam, Toxicity study. |
| Subjects: | AYUSH > Nanju Noolum Maruthuva Neethi Noolum |
| Depositing User: | Subramani R |
| Date Deposited: | 14 Mar 2020 05:58 |
| Last Modified: | 14 Mar 2020 05:58 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/12267 |
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