Standardization on Purification Processes of Umaththai Seeds: A Comparative Analysis

Thusiyanthan Kalaichelvi, (2019) Standardization on Purification Processes of Umaththai Seeds: A Comparative Analysis. Masters thesis, Government Siddha Medical College, Palayamkottai.

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Abstract

Standardization of Siddha drugs, herbal formulations and plant materials is the need of the day. Many of them do not have standard identification tests (or) analytical procedures to maintain their consistent quality. Modern methods describing the identification and quantification of Biomark’s in plants may be useful for proper Standardization of herbs. Based on the above reason, the present study is carried out with an aim of to Standardize the purification process on Umaththai seeds (Datura metal ) based on some qualitative and quantitative analysis as per WHO guidelines. Unpurified sample of Umaththai seed (Karu Umaththai ) is collected from the field of reserved forest of Courtalam hills area. The collected seeds separated by removing the unwanted materials. After drying seeds are shade dried for several days. It preserve in the air tight container. Then the drug is purified as per method suggested in Sarakku Suththisei Muraikal study is carried as per prescribed procedure. PHYSICO CHEMICAL ANALYSIS: • In Physico chemical the pH of unpurified Umaththai seeds is 5.10, it is increased to 6 in purified Umaththai seeds. It shows the safety of drug. • Water soluble extract of unpurified Umaththai is 8.49 it is reduced to 2.36 in purified one. It denotes the prolong the duration of a drug action. • Loss of drying of unpurified Umaththai seeds is found 9.27 it is reduced to 9.16 to purified. It is found within acceptable limit. • Water soluble extract of unpurified Umaththai seeds is 13.3% it is reduced to 6.68% in purified one. It denote the prolong the duration of a drug action. • Total ash of unpurified Umaththai is 2.36 it is redused to 1.77 in purified Umaththai . It is denote the Purity of drug. PHYTOCHEMICAL ANALYSIS: Phytochemical screening result shows the Terpenoids, Glycosides, Alkaloids, Coumarin. Fixed oil are present in all extracts of Datura metal seeds. BIOCHEMICAL ANALYSIS: Copper, Chloride, Nitrate, Sulphate, Floride, Starch, Alkaloid and amino acids are present in all three samples. HEAVY METAL ANALYSIS IN UMATHTHAI SEEDS: Heavy metal analysis is carried out in Umaththai seeds before and after purification by AAS. It is confirmed that the heavy metals are found to be within normal limits on all samples. AFLOTOXIN: Aflatoxin level are quantitatively measured in purified sample, the result shows the absence of them. INTERPRITATION OF PESTICIDE RESIDUE: Pesticide residue are quantitatively measured in purified sample, the result shows the Below Limit of Quantification. TLC/ HPTLC ANALYSIS: Chloroform extract of the seeds shows more absorbance as compared to alcohol extract and the seeds purified by using Lemon juice shows more positive results as compare to others. Thus the chloroform extract of lemon juice at 366 and 575 nm shows more absorbance reveals that, the presence of more active compounds. CONCLUSION: This study shows that standardization on purification process of Umaththai seeds (Datura metal seeds) is done with respect to purification process definitely an important role in making a drug to act without causing any side effects or adverse effect. The Datura seeds are toxic owing to the presence of alkaloids hyoscine, scopolamine and atropine. In the present study, an attempt has been made to study the HPTLC profiling of purified seeds. The purification of Datura metel seeds is done by the Siddha Process “Suddhi” using Lemon juice (DM II) and butter milk (DM III). Different physiochemical parameters are studied before and after purification. HPTLC results shows that, mother tincture of the chloroform extract of the seeds shows more absorbance as compared to alcohol extract and the seeds purified by using Lemon juice shows more positive results as compare to others. Thus the chloroform extract of lemon juice at 366 and 575 nm shows more absorbance reveals that, the presence of more active compounds. Aim of the purification is to minimize the toxic effect of the drug and enhance the potency and safety of a drug. These findings are strongly confirmed the effectiveness of Siddha purification. This is the reason why Siddhars have said purification is must before going to any preparation. In future, in order to strengthen the purification process, the study has shown a better way and will lead to further research in the same area.

Item Type: Thesis (Masters)
Additional Information: (Reg.No.321616009)
Uncontrolled Keywords: Standardization, Purification Processes, Umaththai Seeds, Comparative Analysis.
Subjects: AYUSH > Nanju Noolum Maruthuva Neethi Noolum
Depositing User: Subramani R
Date Deposited: 14 Mar 2020 04:53
Last Modified: 14 Mar 2020 04:53
URI: http://repository-tnmgrmu.ac.in/id/eprint/12262

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