Clinical Role of Micro RNA (miRNA) Expression Profiles in Oral Squamous Cell Carcinomas

Rajkumar, K S (2014) Clinical Role of Micro RNA (miRNA) Expression Profiles in Oral Squamous Cell Carcinomas. Masters thesis, Kilpauk Medical College, Chennai.


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INTRODUCTION: Head and Neck Squamous Cell Carcinoma (HNSCC) include squamous epithelial cancers of the upper aerodigestive tract. HNSCCS represent the sixth most common cancer in the world, and accounts for more than 500,000 new cases annually. HNSCC, especially oral cancers have a multifaceted aetiology. A plethora of lifestyle, environmental, viral and genetic factors has been identified as the risk factor for oral cancers. However, smoking, tobacco chewing, and alcohol consumption are widely considered to be major preventable risk factors. AIM OF THE STUDY: 1. To assess the feasibility of extracting miRNA in oral cancer tumour tissue samples. 2. To assess selected miRNAs expression profiles and compare it with epidemiological data, tumour characteristics and clinical behaviour of oral cancer patients. MATERIALS AND METHODS: Study Design: Prospective collaborative clinical and molecular research Place of Study: 1. Department of Surgical Oncology, Centre for Oncology Govt. Royapettah Hospital & Kilpauk Medical College, Chennai 2. Department of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai. Duration of Study: October 2011 – December 2013. Number of Patients: 36 patients with oral squamous cell carcinomas. Inclusion Criteria: 1. Patients of age 18 to 70 years, 2. Oral squamous cell carcinomas, all sub sites, 3. Patients on regular follow-up, Exclusion Criteria: 1. Metastatic disease, 2. Recurrent or second primary cancers, 3. Patients who had previously undergone any treatment for the primary disease, 4. Other medical illness(es) precluding full study participation. Materials: 1. Clinical data of patients, 2. Tumour tissue samples from biopsies and surgical specimens. RESULTS: Thirty six patients with good quality RNA in the tumour tissue were selected for miRNAs analysis. MiRNAs Expressions of four miRNAs relevant to oral cancer based on available literature (miR-125b2, 184, 21 and 138) were analysed and compared with Clinical data of these 36 patients Epidemiology and Tumour Characteristics: Te male: female ratio was 25:11. The mean age of the patients was 51 years (SD±11, range: 26-70 years). Tongue (n=14, 39%) and buccal mucosa (n=8, 25%) were the most common sub sites. Left sided cancers were more common. Tobacco usage (61 %) in the form of cigarettes, beedis, smokeless tobacco like pan, etc was common. The other addictions seen were betel nut (27.8%), alcohol (33.3%), hans and cocaine. Locally advanced (T4) lesions constituted 47.2 percent of all tumours and node positivity was seen in 70 percent of patients. Using a three grade grading system, 44 percent of tumours were moderately differentiated and 19 percent of tumours were poorly differentiated. SUMMARY AND CONCLUSIONS: The present study was a pilot study of a patient centric analysis of the use of miRNAs expression profiles in the assessing clinical behaviour of oral cancer patients. The study included analysis of 4 select miRNAs (miR - 21, 125 b2, 184 and 138) Expression Profiles in 36 oral cancer patients and their impact on the epidemiological and clinical characteristics of these patients. Based on this study the following conclusions can be made: 1. The extraction and analysis of miRNAs expressions from oral cancer tumour tissue sample is feasible provided care in taken in transportation, storage and processing of the samples. 2. MiRNAs altered expressions have definite and significant associations with many clinical and epidemiological characteristics of oral cancer patients. Development of newer, simpler, more efficient, safer and cost effective techniques for storage, transportation, extraction and analysis of RNA is the need of the hour. Further studies involving a panel of miRNAs are needed to provide conclusive evidences for use of miRNAs in assessing clinical behaviour of oral cancer patients and to influence their management.

Item Type: Thesis (Masters)
Additional Information: Reg.No.181114153
Uncontrolled Keywords: Clinical Role, Micro RNA (miRNA) Expression Profiles, Oral Squamous Cell Carcinomas.
Subjects: MEDICAL > Surgical Oncology
Depositing User: Subramani R
Date Deposited: 16 Feb 2020 10:48
Last Modified: 22 Feb 2020 00:02

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