Vennila, K (2016) To evaluate the preclinical safety and efficacy of Siddha Single herbal formulation Sengathaari Root Bark decoction used in the management of Karappan (Eczema - Contact Dermatitis). Doctoral thesis, The Tamilnadu Dr. M.G.R. Medical University, Chennai.
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Abstract
Eczema and contact dermatitis are the most frequently occurring diseases in pediatric group. Contact dermatitis is most often seen around the hands or body parts that touched or exposed to the irritant/allergen. Contact Dermatitis is an inflammatory process in the skin caused by an exogenous agent that directly or indirectly injure the skin. Among them, an herbal Sastric formulation “Sengathaari Root Bark decoction (SRB)” has been practiced a long time for treating Eczema in southern part of India. SRB has been chosen for the study to prove it is a safe and efficacious drug in the management of Eczema in animal model. The sample was analyzed for qualitative and quantitative estimation. Preliminary physical parameters such as total ash value, moisture content and extractive values were analyzed. The concentration of trace and heavy metals were analyzed using Inductively Coupled Plasma – Optical Emission Spectrometer. From the result of above studies, we inferred that sample was feasible to conduct the study on animal model and clinical trial in human beings. To evaluate the safety of SRB, Wistar albino rats were used for performing acute and 28 days repeated oral toxicity studies following OECD guidelines. 5000 mg/kg (10 ml/kg) of SRB Decoction powder was tested on six rats and observed nil mortality and morbidity. The 28 days repeated oral toxicity was observed on Wistar rats by giving SRB at three dose levels (Low, Mid, High) for 28 days. No mortality and abnormal clinical signs were observed during 28 days. Haematological and biochemical parameters were within the normal limit. No significant abnormality was detected in necropsy study on organs and in H&E sliced organs. Carrageenan and Cotton pellet granuloma method was performed on rat and observed SRB decoction has significant anti-inflammatory activity. Anti-histaminic activity of SRB was performed on mice and observed SRB has significant anti histaminic activity. SRB decoction showed significant anti eczematous effect. IL-2, TNFα gene expressions in quantitative PCR was down regulated in SRB decoction. CONCLUSION: Sengathaari Root Bark which is being widely used for karappan especially in paediatric age group in Southern part of India. After analyzing the physicochemical characterization of SRB Decoction powder and its toxicity and pharmacological studies on animals, it is proved that SRB Decoction is safe and efficacious drug in treating Eczema. Further, the clinical safety and efficacy can be tested by undertaking clinical trial.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | preclinical safety and efficacy, Siddha Single herbal formulation, Sengathaari Root Bark decoction, management, Karappan (Eczema - Contact Dermatitis). |
| Subjects: | AYUSH > Kuzhanthai Maruthuvam |
| Depositing User: | Subramani R |
| Date Deposited: | 14 Jan 2020 17:57 |
| Last Modified: | 15 Oct 2022 12:15 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/11840 |
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