Sudha, M (2016) In Vitro Antioxidant and Free Radical Scavenging activities of Various Solvent Extracts of Marsdenia Brunoniana. Doctoral thesis, The Tamilnadu Dr. M.G.R. Medical University, Chennai.
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Abstract
The various solvent extracts of Marsdenia brunoniana was prepared and evaluated for its in vitro antioxidant, free radical scavenging and in vitro alpha glucosidaseand alpha amylase inhibitory activities. Amongst the various solvent extracts of M.brunoniana, ethanol extract shows more pronounced activity while comparing with remaining solvent extracts. Hence, the ethanolic extract of Marsdenia brunoniana has been studied for the toxicological, antidiabetic, antihyperlipidemic and nephroprotective activities in STZ-NAA induced diabetic animals. And the acute toxicity results revealed that the extract did not produce any motility to toxic effects even at maximum dose administered. Subacute toxicity study also explored the non-toxic nature of the extract. Further, the extract was subjected to various studies like glucose uptake assay, DNA protection assay and in vitro cytotoxic assay. The results obtained in these studies revealed that the extract is significantly increases the glucose uptake and it protects the DNA and it also did not produce any cytotoxic activity at the tested dose levels. The Preliminary phytochemical analysis, HPLC, HPTLC and GC-MS analysis of EEMB also revealed the presence of many phytochemical compounds such as flavonoids, phenols, methyl-α-D-Glucopyranoside, 4-C-methyl-Myo-Inositol, n-hexadecanoic acid, phytol, rutin, quercetin, ferulic acid, caffeic acid, gallic acid and which are having pronounced biological effects. The EEMB significantly reduces the elevated level of glucose in blood and level of lipid profile in diabetic persuaded animals. At the same time it is able to increase the insulin secretion, restore the antioxidant enzyme system and prevent the weight loss in diabetic animals. It also has the incredible effect on reducing the glycosylated hemoglobin level in diabetic induced animals.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | In Vitro Antioxidant, Free Radical Scavenging activities, Various Solvent Extracts, Marsdenia Brunoniana. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Subramani R |
| Date Deposited: | 14 Jan 2020 16:39 |
| Last Modified: | 15 Oct 2022 13:34 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/11829 |
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