Bioanalytical Method Development and Validation of Selected Corticosteroids in Rat Plasma using RP-HPLC Method

Nathe Kiran Ramesh, (2017) Bioanalytical Method Development and Validation of Selected Corticosteroids in Rat Plasma using RP-HPLC Method. Masters thesis, RVS College of Pharmaceutical Sciences, Coimbatore.


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This dissertation deals with the studies carried out by the writer in this laboratory on the development of bioanalytical method used for the study of validation parameters of selected corticosteroids in rat plasma by RP-HPLC method. Before discussing the experimental results, a brief introduction to bioanalytical methods of analysis, biopharmaceutical analysis, analysis of drugs in biological media, preliminary treatment of biological samples, estimation procedures for drugs and metabolites from biological samples, estimation of drugs in biological sample by RP-HPLC or LC-MS/MS methods. A simple, rapid and sensitive bioanalytical method for quantitative estimation of corticosteroids such as Betamethasone, Dexamethasone and Prednisolone in rat plasma using RP-HPLC method has been developed and validated and results obtained By analyzing these above selected drugs (Betamethasone, Dexamethasone and Prednisolone) and validating with the parameters we conclude that they showed excellent recovery and reproducibility in all aspects comparing with reported methods. Hence the developed method will be much useful to analyze plasma samples in rat/human, which will be more effective when compared with the reported methods. • The current validated bioanalytical RP-HPLC method for Betamethasone, Dexamethasone & Prednisolone offers good accuracy and significant advantages in terms of linearity, stability & selectivity. • The separation method developed produce acceptable values of recovery. The chromatogram developed has well resolved peaks of above selected corticosteroids without any interference. • By using current developed method we can get maximum absorptivity of selected drugs as compared to earlier reported methods. • The developed method is most economical as compared to earlier methods due to use of Methanol as a solvent. • In this method I try to maintain minimum retention time (RT) with consideration of plasma peaks.

Item Type: Thesis (Masters)
Additional Information: Reg.No.141540002
Uncontrolled Keywords: Bioanalytical Method Development, Validation, Corticosteroids, Rat Plasma, RP-HPLC Method.
Subjects: PHARMACY > Pharmaceutical Chemistry
Depositing User: Subramani R
Date Deposited: 24 Sep 2019 08:55
Last Modified: 24 Sep 2019 08:57

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