A Study of Chromosomal Abnormalities in Leukemic Patients in a Tertiary Care Hospital

Ramesh, B (2019) A Study of Chromosomal Abnormalities in Leukemic Patients in a Tertiary Care Hospital. Masters thesis, Madras Medical College, Chennai.


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BACKGROUND: Leukemias are hematopoietic malignant neoplasms arising by the clonal expansion of the hematopoietic stem cell and can be divided into acute and chronic leukemias. AIMS AND OBJECTIVES: 1. To determine the chromosomal abnormality associated with each type of leukemia. 2. To study the distribution of karyotype, in relation to the hematological parameters like total leucocyte count, platelet count and hemoglobin levels. MATERIALS AND METHODS: A total of 30 cases are taken for the study. This is a prospective study conducted for a period of 1.5 years, with the study centre being Institute of Pathology, Rajiv Gandhi Government General Hospital. OBSERVATION AND RESULTS: 13 cases had CML morphology on smear, of which 12 cases had the BCR-ABL translocation and 1 case was Ph negative. 10 cases had the preponderance of myeloblasts on smear and diagnosed as AML,with different karyotypes. 4 cases had the preponderance of lymphoblasts in peripheral smear and diagnosed as ALL. All 4 cases had different karyotypes. There were 2 cases of CLL with normal karyotype and 1 case of biphenotypic leukemia. CONCLUSION: Conventional cytogenetics is a cost-effective, simple technique that allows an overall view of the genome. This can be combined with other molecular methods like florescent in situ hybridization (FISH), which is essential to enable the detection of cryptic rearrangements and PCR, which helps in quantification of the mutated protein.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Acute myeloid leukemia (AML), Chronic myeloid leukemia (CML), Chronic lymphoid leukemia (CLL), Acute lymphoid leukemia (ALL), Karyotyping, translocation, deletion, Philadelphia.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 24 Aug 2019 10:49
Last Modified: 24 Aug 2019 10:49
URI: http://repository-tnmgrmu.ac.in/id/eprint/11172

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