The Effect of Histamine on Exocytosis in Bovine Chondrocytes

Mahatabb Nundy, (2019) The Effect of Histamine on Exocytosis in Bovine Chondrocytes. Masters thesis, Christian Medical College, Vellore.


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BACKGROUND: Osteoarthritis involves cartilage damage. Chondrocytes are known to secrete matrix metalloproteinases (MMP). It is also known that osteoarthritic synovial fluid contains high levels of histamine (from sources like mast cells). The aim of the study was to analyze a possible role for histamine in chondrocyte exocytosis. OBJECTIVES: 1. To investigate the role of histamine on chondrocyte secretion. 2. To identify the histamine receptors on bovine chondrocytes. 3. The study effect of the absence or presence of intracellular ATP on exocytosis. METHODS: Bovine chondrocytes were sourced from the local slaughter house. Cartilage shavings were obtained from these and digested. Chondrocytes were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) with Ham’s F12 additive, ascorbic acid and L-Glutamine. The isolated cells were then used for patch clamp studies. Capacitance of cell membrane was recorded. An increase in capacitance indicates a net increase in cell surface area and therefore a net exocytosis. The effect of histamine on cellular capacitance was recorded. RESULTS: Cell membrane capacitance was found to increase in the presence of histamine. H1 and H2 receptors are found to be present in bovine chondrocytes. Exocytosis though was possible only in the presence of ATP in the intracellular fluid. CONCLUSION: Exocytosis of matrix degrading enzymes has been observed in osteoarthritic joints. Several studies have shown that histamine levels are also significantly high in the same. From our study we conclude that histamine does induce exocytosis. This might open new avenues of treatment of arthritis.

Item Type: Thesis (Masters)
Additional Information: Reg.No.201615354
Uncontrolled Keywords: patch clamp, capacitance, exocytosis, histamine.
Subjects: MEDICAL > Physiology
Depositing User: Subramani R
Date Deposited: 23 Aug 2019 02:01
Last Modified: 23 Aug 2019 02:01

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