Evaluation of the Level of Coagulation Factors V and VIII on Storing Fresh Frozen Plasma at Different Temperatures: A Study at Regional Blood Bank and CEmONC Centre

Sakthipriya, B (2019) Evaluation of the Level of Coagulation Factors V and VIII on Storing Fresh Frozen Plasma at Different Temperatures: A Study at Regional Blood Bank and CEmONC Centre. Masters thesis, The Tamilnadu Dr. M.G.R. Medical University, Chennai.


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BACKGROUND: Fresh frozen plasma forms the backbone in the management of coagulopathies due to liver disease, disseminated intravascular coagulation, Thrombotic thrombocytopenic purpura, Coumadin use and major blood loss. Wastage of this resource should be avoided or minimized. Hence we conducted this prospective study to analyse the stability of coagulation factors in thawed plasma that was previously frozen at two different temperatures and now stored at 2°C to 6°C over 5 days. The study was done at The Department Of Transfusion Medicine, The Tamilnadu Dr. MGR Medical University, Guindy, Chennai and at Govt. Kilpauk Medical College & Hospital, Chennai. OBJECTIVES: 1. To analyse the coagulation factors V and VIII levels in fresh frozen plasma stored at -30°C and at -70°C for a duration of 3 months. 2. To evaluate whether the thawed plasma stored for a considerable time at 2°C to 6°C is appropriate for therapeutic use. MATERIALS AND METHODS: Forty samples of fresh plasma were collected, baseline values of fibrinogen, factor V, factor VIII, PT and APTT measured and were then divided into two groups, frozen and stored at -30°C and -70°C respectively for 3 months. They were then thawed at 37°C, and stored at 2°C to 6°C for 5 days. The parameters were measured at regular intervals during the period of storage of thawed plasma. OBSERVATIONS: In both the groups, even the coagulation values measured immediately after thawing (day 0) showed a statistically significant difference compared to baseline. This significance extended up to day 5. However, all the values of observed parameters were within the physiological limits. This shows that the process of storage of Fresh Frozen Plasma does cause a decrease in the coagulation factors immaterial of the storage temperature, though within therapeutic range. Between the groups, the -70°C group performed better in retaining the stability of coagulation factors. This was evident in the statistically significant difference observed between the groups at 72 hours and 120 hours after thawing and storage at 2 to 6°C. The values observed in the -30°C group was however in the hemostatic range. Among the factors, fibrinogen was found to be the most stable with lesser fluctuations from the baseline values. Factor VIII was observed to be the most labile among the observed parameters, decreasing by 28% from the baseline value. Yet, the therapeutic measure of atleast 0.5 units/ml as suggested by the European Pharmacopoeia was not breached. CONCLUSION: We conclude that the coagulation factors in thawed plasma that was frozen previously at -30°C and -70°C, retained its stability when stored at 2°C to 6°C for 5 days. After thawing, if stored at appropriate temperature, the plasma can be utilized up to 5 days, thereby minimizing its wastage.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Fresh frozen plasma, Thawed plasma, fibrinogen, Factor V, Factor VIII, Prothrombin time, activated partial thromboplastin time.
Subjects: MEDICAL > Immunohaematology Blood Transfusion
Depositing User: Subramani R
Date Deposited: 19 Aug 2019 00:59
Last Modified: 19 Aug 2019 00:59
URI: http://repository-tnmgrmu.ac.in/id/eprint/10933

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