Analysis of Red Complex Bacteria in the microbiome of whole Saliva in Periodontal Health and Periodontitis Individuals using Next Generation Sequencing Technology

Kavipriya, K (2019) Analysis of Red Complex Bacteria in the microbiome of whole Saliva in Periodontal Health and Periodontitis Individuals using Next Generation Sequencing Technology. Masters thesis, Ragas Dental College and Hospital, Chennai.

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Abstract

BACKGROUND: The red complex bacteria which includes Porphyromonas gingivalis, and Tannerella forsythia (formerly Bacteroides forsythus), Treponema denticola are described as climax colonizers and are thought to be play an important pathogen in progression of periodontal disease. These bacterial species are usually found together in periodontal pockets, suggesting that they may cause destruction of the periodontal tissue in a cooperative manner. Technological developments in sequencing and identifying DNA and powerful bioinformatics tools have helped in characterization of microbia of interest in relation to the total microbial load. AIM OF THE STUDY: The aim of our study was to evaluate the presence of the red complex bacteria in saliva using Next Generation Sequencing Technology in periodontal health and periodontitis. MATERIALS AND METHODS: A total of 20 individuals seeking dental treatment in Ragas Dental College and Hospitals, Chennai, were involved in the present study, of which 10 were periodontally healthy individuals (control group) and 10 were periodontitis patients (test group). The salivary red complex was investigated with NGS technology using Illumina MiSeq sequencing method. Amplicons from V3-V4 hypervariable regions of 16S rRNA gene were sequenced. RESULTS: The results of our study suggest that the Red complex bacteria were significantly higher in periodontitis [P. gingivalis (

Item Type: Thesis (Masters)
Uncontrolled Keywords: Illumina MiSeq Sequencing, Next Generation Sequencing, 16S rRNA, Periodontitis, Dysbiosis, salivary microbiome.
Subjects: DENTAL > Periodontology
Depositing User: Subramani R
Date Deposited: 15 Aug 2019 02:58
Last Modified: 15 Aug 2019 02:58
URI: http://repository-tnmgrmu.ac.in/id/eprint/10880

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