Ruth Vijitha, B (2019) Identification of Candida Auris in Immunocompromised Patients, Denture wearers and in Normal Individuals using Real Time-Polymerase Chain Reactions (RT-PCR). Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: C.auris with a highly divergent genome is an emerging etiologic agent of fungemia. C.auris isolated in India was found to be resistant to fluconazole and genotypically distinct from isolates from South Korea and Japan. There is an urgent need for accurate fungal screening methods such as PCR to ensure proper management of fungemia. C.auris fungemia is associated with a high mortality rate (66%).C.auris has a highly divergent genome. AIM AND OBJECTIVE: To identify the presence of C.auris in the saliva of immunocompromised patients, denture wearers and normal individuals by quantitative real time polymerase chain reactions (RT- PCR/q PCR). MATERIAL AND METHODS: Unstimulated saliva was collected from 10 HIV patients, 10 denture wearers and 10 normal individuals. In order to identify the quantitative presence of C. auris, 10ng of total DNA isolated from the saliva of HIV patients, denture wearers or normal control subjects was subjected to real time PCR. RESULTS: The results were obtained in amplification curve. Samples with higher concentration of C.auris specific signal showed early Ct values, while samples with lower concentration of C.auris specific signal showed delayed Ct values. Saliva samples of denture wearers showed higher content of high copy numbers of C.auris. CONCLUSION: Molecular methods are accurate and rapid method of identification of C.auris. Real Time Polymerase Chain Reaction (RT-PCR) proved to be highly specific and sensitive method of identification of the rare nosocomial pathogen C.auris.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Candida auris, RT-PCR, Cycle Threshold. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 12 Aug 2019 15:19 |
| Last Modified: | 12 Aug 2019 15:19 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10788 |
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