Pradeepsankar, S (2019) Immunohistochemical Expression of p63 and β-catenin in Different Grades of Oral Squamous Cell Carcinoma. Masters thesis, Adhiparasakthi Dental College and Hospital, Melmaruvathur.
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Abstract
BACKGROUND: Oral squamous cell carcinoma (OSCC) results from the multistep accumulation of heterogenous genetic changes. The poor prognosis of oral cancer is due to several reasons such as late stage diagnosis, low response to current therapeutic strategies, primary site recurrence, high rate of metastasis to regional lymph nodes etc. which has strongly recommended the need to improve the diagnostic capabilities. Assessing the tumor behaviour at the molecular level will help in initiating appropriate management there by reducing the morbidity associated with malignancy. Two important molecular factors that is known to be associated with cell proliferation and maintaining cell junction are p63 and β -Catenin respectively. AIM OF THE STUDY: To evaluate the expression of p63 and β-catenin in different grades of oral squamous cell carcinoma using immunohistochemistry. MATERIALS AND METHODS: A total of 36 samples were examined for the immunohistochemical expression of p63 and β-catenin. The control group includes 15 formalin fixed par affin embedded tissue blocks of normal buccal mucosa. The study group includes 21 cases of formalin fixed paraffin embedded tissue blocks of oral squamous cell carcinoma (10 cases of well differentiated and 11 case of moderately differentiated squamous cell carcinoma). 3 micron thickness sections were made from each sample and stained with p63 antibody and βcatenin antibody. The intensity and area of staining was assessed and scored. The data obtained statistically analysed. RESULTS: Comparison of staining intensity showed a significant difference between normal and well differentiated carcinoma and also between normal and moderately differentiated carcinoma. However, no significant difference in staining intensity was found between well differentiated and moderately differentiated carcinoma. Similarly comparison of area of staining showed a significant difference between normal and well differentiated carcinoma and also between normal and moderately differentiated carcinoma. However, no significant difference in area of staining was found between well differentiated and moderately differentiated carcinoma. Comparison of staining intensity did not show any significant difference between normal and well differentiated carcinoma, between normal and moderately differentiated carcinoma and also between well differentiated and moderately differentiated carcinoma. Similarly comparison of area of staining showed no significant difference between normal and well differentiated carcinoma, between normal and moderately differentiated carcinoma and also between well differentiated and moderately differentiated carcinoma CONCLUSION: We conclude that the expression of both β-catenin and p63 can be used as a prognostic marker in oral squamous cell carcinoma.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Squamous cel l carcinoma, Immunohistochemistry, β-catenin, p63. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 12 Aug 2019 15:18 |
| Last Modified: | 12 Aug 2019 15:18 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10783 |
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