Effect of Calcarea Carbonica on Osteoporotic Activities in Ovariectomised Albino Rat Model of Osteoporosis in 1X, 30 and 0/1 Potencies

Alphy, Mathew (2019) Effect of Calcarea Carbonica on Osteoporotic Activities in Ovariectomised Albino Rat Model of Osteoporosis in 1X, 30 and 0/1 Potencies. Masters thesis, Sarada Krishna Homoeopathic Medical College, Kulasekharam.


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BACKGROUND: From a retrospective study on Calcarea Carb done at Sarada Krishna Homoeopathic Medical College, it is found that Calcarea Carb has great affinity for musculoskeletal system (3). Females between 36-60 years have shown more musculoskeletal affection than any other age groups. This age group mainly includes menopausal women. Menopause can bring about changes in body mass index and can result in osteoporotic changes. In the modern era of evidence based medicine I wish to prove the same experimentally upon ovariectomised albino rats. Ovariectomy can induce menopause and thereby osteoporosis in rats. The study is done using 3 different potencies of Calcarea Carb, i.e, 1X, 30 and 0/1 to see the effectiveness of this remedy in controlling osteoporosis in ovariectomised albino rats. AIMS AND OBJECTIVES: 1. To study the effect of Calcarea Carb, in osteoporotic activity, in ovariectomised rat model of osteoporosis with 3 different potency scales, 1X, 30 and 0/1. 2. To compare the effect of Calcarea Carb with estrogen in controlling osteoporosis. 3. To study the potency that is more suitable in treating osteoporosis in ovariectomised rat model of osteoporosis. MATERIALS AND METHODS: STUDY SETTINGS: 30 female Wistar albino rats are taken for the study. The rats are divided into 5 groups consisting of 6 animals each. SELECTION OF SAMPLES: Healthy female albino rats of about 90 days old, weighing about 150-220g is taken for the study. STUDY DESIGN: This is an experimental study done on ovariectomised albino rats. INTERVENTION: Calcarea Carb is administered orally in 3 different potencies 1X, 30 and 0/1 to 3 different groups of female Wistar albino rats respectively. The remedy is given in water dose using distilled water, i.e., 5 drops, 3 times daily. Freshly prepared dose is administered each time. CONCLUSION: Calcarea Carb is effective in controlling osteoporosis in ovariectomised albino rat model of osteoporosis in 3 different potency scales i.e, 1X, 30 and 0/1. • Calcarea Carb 0/1 is effective in controlling osteoporosis by using the elevated biochemical parameters beneficially for osteoblastic activity. It has also taken care of the bone physically and histologically. So Calcarea Carb 0/1 is the best potency for controlling osteoporosis as per the study. • Calcarea Carb 30 is the next effective potency for controlling osteoporosis, which is evident from the physical, biochemical and histological parameters. • Calcarea Carb 1X due to its physiological or material nature, has elevated the physical and biochemical parameters to the maximum but has not showed remarkable changes in bony architecture. This confirms that its action is supplementary in nature. • The action of Calcarea Carb is comparable to that of estrogen in elevating physical and biochemical parameters and acted more than estrogen in histological parameter and serum osteocalcin parameter especially in dynamic doses of Calcarea Carb 30 and Calcarea Carb 0/1. • p value is less than 0.05 for parameters such as body weight, uterine weight, left femur weight, left femur density and in serum levels of calcium, phosphorous, alkaline phosphatase and osteocalcin, indicating that the test is significant. For left femur volume and left femur length, p value is greater than 0.05, indicating that it is due to chance. SUMMARY: Osteoporosis was induced to a sample size of 30 female Wistar albino rats (divided randomly into 5 groups containing 6 animals each) through ovariectomy after 1 week of acclimatization. After 15 days of post surgical care, these 5 groups received their respective treatment for a period of 90 days after which they were sacrificed for investigations. It was found that among the homoeopathic potencies Calcarea Carb 0/1 showed highest result in osteoblastic activity by effectively using the calcium, phosphorous and alkaline phosphatase for bone formation. Calcarea Carb 30 was found to be the next effective potency in controlling osteoporosis. It is followed by Calcarea Carb 1X. All the 3 potencies are found to be effective in controlling osteoporosis by taking care of the bone physically, biochemically and histologically. Calcarea Carb has also saved the uterus from post menopausal atrophy and helped in maintaining the body weight. Comparative study with the control group and estrogen group confirmed the same. The tests are significant, with a p value < 0.05, for all the parameters except for left femur length and left femur volume; where it is due to chance.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Osteoporosis, Ovariectomy, Calcarea Carbonica, Osteocalcin, Alkaline phosphatase, Calcium, Phosphorous.
Subjects: AYUSH > Materia Medica
Depositing User: Subramani R
Date Deposited: 05 Aug 2019 01:50
Last Modified: 27 Mar 2020 14:08
URI: http://repository-tnmgrmu.ac.in/id/eprint/10743

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