Ion Channels in Peripheral Blood Mononuclear Cells – Effect of Culture and Ascorbic Acid.

Snekalatha, S (2014) Ion Channels in Peripheral Blood Mononuclear Cells – Effect of Culture and Ascorbic Acid. Doctoral thesis, Christian Medical College, Vellore.

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Abstract

Peripheral blood mononuclear cells which include lymphocytes and monocytes play important role in the adaptive and innate immune responses. Cell mediated immunity is sub served by lymphocytes and innate immunity by granulocytes, monocytes and macrophages. Ion channels on the cell membrane play significant roles during activation of these immune cells The activation of lymphocytes is dependent on function of voltage gated potassium channels, calcium activated potassium channels and calcium release activated channels(CRAC) channels. Similarly, voltage gated proton currents play a key role in the respiratory burst of phagocytic cells. The expression of these ion channels on the membrane may change when the cells are exposed to culture environment in which they may undergo differentiation. Further, the function of these ion channels can be modulated by certain drugs for therapeutic purposes. Drugs may also alter the expression of ion channels on the cell membrane Vitamins act as immunomodulants and are known to alter gene expression in immune cells. The present work involves study of the changes in two ionic currents in peripheral blood mononuclear cells during culture and the effect of vitamin C on these currents when it is present in supraphysiological concentrations in the culture environment. The proton currents in peripheral blood monocytes were characteristically similar to proton currents reported in other mammalian phagocytes in terms of their voltage dependence and gating kinetics. The currents recorded in peripheral blood monocytes were larger than those reported in THP-1 monocytes. The proton current density decreased during culture when the cells differentiated into macrophages or dendritic cells. The changes can be correlated to a certain extent to decreased NADPH oxidase in monocyte derived cells reported earlier, though a functional significance cannot be clearly stated with the experiments done in this study.

Item Type: Thesis (Doctoral)
Uncontrolled Keywords: Ion Channels; Peripheral Blood Mononuclear Cells; Culture and Ascorbic Acid
Subjects: MEDICAL > Physiology
Depositing User: Subramani R
Date Deposited: 18 Jun 2017 14:44
Last Modified: 18 Jun 2017 14:44
URI: http://repository-tnmgrmu.ac.in/id/eprint/107

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