Saurabh Kumar, (2018) Method Development, Validation and Simultaneous Estimation of Quinapril and Hydrochlorthaizide in Tablet Dosage Form by using RP-HPLC. Masters thesis, J.K.K.Nattraja College of Pharmacy, Komarapalayam.
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Abstract
A simple, accurate, precise method was developed for the simultaneous estimation of the Quinapril and HCTZ in Tablet dosage form. Chromatogram was run through Inertsil ODS column (250mm: 4.6mm, 5μ). Mobile phase containing Buffer & Acetonitrie in the ratio of (45:55) was pumped through column at a flow rate of 0.8ml/min. Buffer used in this method was ortho phosphoric acid Temperature was maintained at 30°C. Optimized wavelength for Quinapril and HCTZ was 210nm. Retention times of Quinapril and HCTZ were found to be 2.17min and 3.4min. % RSD of the Quinapril and HCTZ were and found to be 0.65 and 0.42 respectively. % Recover was Obtained as 99.91 and 100.02 for HCTZ and Quinapril respectively. LOD, LOQ values are obtained from regression equations of Quinapril and HCTZ were 0.67, 2.03 and 0.5, 1.52 respectively. Regression equation of HCTZ is y = 20044x + 4078, and of Quinapril is y = 317877x + 2711 Regression co-efficient was 0.999. OBJECTIVE AND PLAN OF STUDY: Literature survey reveals that, there are no simple and cost effictive spectrophotometric and HPLC method available for the estimation of “COMBINATION OF HCTZ AND Quinapril” in bulk and various pharmaceutical dosage forms. The objectives of the present work are, 1. To develop UV-Visible spectrophotometric method for estimation of combination of HCTZ and Quinapril. 2. To develop analytical method for quantitation of combination of HCTZ and Quinapril by RP-HPLC. 3. Validation of all developed analytical methods as per ICH guidelines. 4.The proposed method should be cost effictive & simple method. For Analysis of HTCZ and Quinapril the combained doses form. A simple, accurate, precise method was developed for the simultaneous estimation of the Quinapril and HCTZ in Tablet dosage form. Chromatogram was run through Inertsil ODS column (250mm: 4.6mm, 5μ). Mobile phase containing Buffer & Acetonitrie in the ratio of (45:55) was pumped through column at a flow rate of 0.8ml/min. Buffer used in this method was ortho phosphoric acid Temperature was maintained at 30°C. Optimized wavelength for Quinapril and HCTZ was 210nm. Retention times of Quinapril and HCTZ were found to be 2.17min and 3.4min. % RSD of the Quinapril and HCTZ were and found to be 0.65 and 0.42 respectively. % Recover was Obtained as 99.91 and 100.02 for HCTZ and Quinapril respectively. LOD, LOQ values are obtained from regression equations of Quinapril and HCTZ were 0.67, 2.03 and 0.5, 1.52 respectively. Regression equation of HCTZ is y = 20044x + 4078, and of Quinapril is y = 317877x + 2711 Regression co-efficient was 0.999.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg. No. 261630205 |
| Uncontrolled Keywords: | Hydrochlorthiazide (HCTZ), Quinapril, Reverse Phase-High Performance Liquid Chromatography (RP-HPLC). |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Subramani R |
| Date Deposited: | 30 Jun 2019 13:29 |
| Last Modified: | 30 Jun 2019 13:29 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10672 |
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