Ansa, P U (2013) Partial Purification of Anti-Tumor and Antioxidant Components from Uvaria Narum (Dunal) Wall Seed. Masters thesis, RVS College of Pharmaceutical Sciences, Coimbatore.
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Abstract
Uvaria is a genus of flowering plants in the soursop family (Padmaja et al.,1993), Annonaceae. The generic name Uvaria is derived from the Latin uva meaning grape, because the edible fruits of some species in this genus resemble grapes. Scandent shrubs, branchlets sparsely hairy. It is a large climbing shrub with woody stems. Uvaria is a large woody stellately pubescent straggling shrub with dark bluish green leaves. Uvaria narum (Tran et al., 2013) is mainly used in eczema, itching, varicose vein, haemorrhoids, jaundice, inflammation and fever. Leaves are recommended in rheumatic swellings, jaundice, biliousness and fevers. Root is used in the treatment of jaundice, fever, biliousness and typhoid. A decoction of the root bark is given to women to control fits at the time of delivery. It is also used in rheumatism, bowel complaints of Children, for eczema and used in skin diseases. Oil Extracted from the root reduces burning sensation of the Liver. The whole root extract of Uvaria narum could be medicinally employed as a possible free radical scavenger. The plant may also be considered against ageing and other disease caused by free radicals. The aerial parts of Uvaria are used in stomach disorder. OBJECTIVES: 1. Preparation of oil extract and its fractions of Uvaria narum seed. 2. Screening of Phytochemical constituents of seed oil extract using standard assays as well as purification by Thin Layer Chromatography. 3. Analysis of antioxidant activity of seed oil extract and its selected fractions using various In vitro assays. 4. Analysis of In vivo antitumor activity of oil extract of seeds of Uvaria narum in mice using DLA induced solid tumor model. CONCLUSION: The seed oil fraction of Uvaria narum possesses significant cytotoxic effect towards malignat cells than normal cells. In the present study two cytotoxic fractions one positive to alkaloid stains and the other positive to anthraquinone in TLC could be purified. Both these fractions exhibited siginificant cytotoxicity towards tumor cells however they are less toxic to normal cells. The alkaloid fraction reduce DLA induced tumor progression in mice but does not show weight loss, decrease in WBC count and haemoglobin level in the tumor bearing animals suggesting a promising antitumor property. The other two fractions, Fraction-III (positive to terpenoids) and Fraction-IV (positive to tannins and phenolics) are also obtained from this seed oil extract of Uvaria narum of which Fraction-IV exhibits significant antioxidant potential. Overall partial purification of promising antitumor alkaloids and antioxidant tannin/phenolic has been achieved from Uvaria narum seeds. It is expected that further level of purification of these fractions may yield pharmacologically important phytochemicals.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No.26116393 |
| Uncontrolled Keywords: | Partial Purification ; Anti-Tumor ; Antioxidant Components ; Uvaria Narum (Dunal) Wall Seed. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Subramani R |
| Date Deposited: | 30 Jun 2019 12:30 |
| Last Modified: | 30 Jun 2019 12:30 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10668 |
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