Vinciya, T (2018) Evaluation of Antiurolithiatic Activity of Ethanolic and Aqueous Extract of Phaseolus Vulgaris Linn Seeds. Masters thesis, Ultra College of Pharmacy, Madurai.
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Abstract
Kidney stones are hard deposits of minerals like calcium either in urinary tract (urolithiasis) or in kidneys (nephrolithiasis). Along with plenty of fluid intake and urinary retention, the management of urolithiasis also includes combined surgical and medical approach using percutaneous nephrolithotomy (PCNL), extracorporeal shock wave lithotripsy (ESWL) and antibiotics. Surgical treatments are relatively costly, painful and require expert hand and availability of appropriate equipment. This has led to a trigger in search of natural resources possessing antiurolithiatic activity. In spite of tremendous advances in the field of medicine, there is no truly satisfactory drug for the treatment of renal calculi. Most patients still have to undergo surgery to rid of this painful disease. Many remedies have been employed during ages to treat renal stones. Most of remedies were taken from plants and proved to be useful, though the rationale behind their use is not well established except for a few plants and some proprietary composite herbal drugs and they are reported to be effective with no side effects. Hence the search for antilithiatic drugs from natural sources has assumed greater importance. The literature search reveals the potential of several plant drugs such as seeds of Trachyspermum ammi (L) Sprague ex (umbelliferae), seeds of Armoracia lopathifolia (Brassicaceae), rhizomes of Bergenia ligulata (Saxifragaceae), seeds of Agrimonia eupatoria L( fabaceae) in managing Lithiasis in animal models. Phaseolus vulgaris, also known as kidney beans, is a common Indian dish. The seeds of Phaseolus vulgaris are gaining increasing attention as a functional or nutraceutical food, due to its rich variety of phytochemicals such as proteins, amino acids, complex carbohydrates, dietary fibers, oligosaccharides, phenols, saponins, flavonoids, alkaloids and tannins, with potential health benefits. The seeds were claimed to possess diuretic activity and were commonly used in water retention treatment in pregnant women. Studies indicate that seeds of Phaseolus vulgaris were found to have activities such as enhancement of the bifidogenic, antioxidant, antimutagenic, anticarcinogenic and antihyperglycemic effects. Phaseolus vulgaris Linn an easily available plant in India has been explored for the possession of analgesic and anti-inflammatory activity, Antioxidant activity, Anti-obesity, Antibacterial, Anticancer, Antidiabetic, Antifertility, Hepatoprotective, Hypolipidemic, Scope and plan of work Dept of Pharmacology, Ultra College of Pharmacy. Trypsin and α-amylase inhibitor, Hyperglycaemic activity, Low nitrate stress, Anti tubular activity, Atypical anti-psychotic, and Anti-fungal activity. The present study is aimed at evaluating the antiurolithiatic activity of the ethanolic extract and aqueous extracts of the seeds of Phaseolus vulgaris was investigated in Wistar rats against Calcium oxalate urolithiasis induced by ethylene glycol (EG) and ammonium chloride (AC) in drinking water. The recurrence of urolithiasis represents a serious problem, as patients who have formed a stone are more likely to form another, and thus stone prevention is highly recommended. Therefore the prophylactic use of the extracts in urolithiasis is also evaluated in the study. OBJECTIVES: 1. To prepare aqueous and ethanolic extracts of Phaseolus vulgaris seeds and characterise its phytochemical nature by preliminary screening, TLC and quantification of some phyto constituents. 2. To evaluate the anti-oxidant action of Phaseolus vulgaris in 3 invitro models. 3. To investigate the anti urolithiatic activity of Phaseolus vulgaris in wistar albino rats by preventive and curative studies. CONCLUSIONS: The results of the present investigation on the evaluation of antiurolithiatic activity of aqueous and ethanolic extracts in rat models of preventive and curative lithiasis have led to the following conclusions. • In preventive and curative studies of AEPV and EEPV showed significant reduction in calcium, oxalate and phosphate levels in urine & an increase in the urinary magnesium and a restoration of normal urine volume. Serum levels of creatinine, uric acid and BUN were also brought down to normal values by the extracts. Histopathological observations also confirm the same. These findings indicate the potential of the extracts in inhibiting kidney stone formation as well as a lithotripsic action on the formed renal stones. • Aqueous extract is more potent than the ethanolic extract in the urolithiasis rat models. • The promising antioxidant activity of the extracts as revealed by in vitro nitric oxide scavenging assay, reducing power assay and in vitro lipid peroxidation study would have contributed to the antiurolithiatic activity of the extracts. • Flavonoids, Polyphenols and saponins present in the extracts may be responsible of the antioxidant and antilithiatic activity of the extracts.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Phaseolus Vulgaris Linn Seeds ; Evaluation ; Antiurolithiatic Activity ; Ethanolic and Aqueous Extract. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Subramani R |
| Date Deposited: | 28 Jun 2019 17:45 |
| Last Modified: | 28 Jun 2019 17:45 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10662 |
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