Sudha, M (2013) Evaluation of Potential Central Protective Role of Ethanol Extract of Pedalium Murex Linn. In Acute and Chronic Unpredictable Stress (CUS) Induced Models in SD Rats. Masters thesis, J.K.K.Nattraja College of Pharmacy, Komarapalayam.
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Abstract
The present study was designed to evaluate the possible protective effect of ethanol extract of Pedalium murex (EEPM) leaves treatment against acute and CUS-induced neurobehavioral models in SD rats. The neuroprotective effect was assessed using a battery of invitro tests, behavioral tests (anxiety, depression and, cognition and memory) and biochemical analysis (SOD, CAT, GSH) of the rat brain. Phytochemical, total phenolic, tannins and flavonoid content of EEPM and, in vitro antioxidant studies on EEPM leaves on various antioxidant systems such as lipid peroxidation, DPPH, nitric oxide, CUPRAC assay, metal chelation and reducing index were performed. Application of acute stress using immobilization for one day and CUS (induced by forced swimming + immobilization + dark phase, for 10 days), resulted in elevated levels of anxiety, depression and changes in memory and cognitive abilities. Pretreatment with EEPM for 30 days prevented the effect of acute and chronic unpredictable stress in rats. The protective effect of EEPM was evaluated for anxiolytic (elevated plus-maze apparatus and depression was evaluated using forced swim test (FST) in both the acute and CUS-induced models and moreover, effect of EEPM on learning and short-term (spatial) memory was evaluated using a radial arm maze (RAM) test. To support the neuroprotective effect of EEPM, the implications of EEPM on general behavior, body weight (BW), food intake (FI), water intake (WI) were undertaken in acute and CUS-induced models, respectively, as these behavior could [Type the document title] Summary and Conclusion Dept. of Pharmacology 130 J.K.K.Nattraja College of Pharmacy be profoundly disturbed in anxiety, depression and may impair memory and cognitive abilities. Also there may be perturbations in the levels of brain antioxidant enzyme system in stressful states. Hence, the effects of EEPM on the levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) were studied in the rat brains.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No: 26113093 |
| Uncontrolled Keywords: | Potential Central Protective Role ; Ethanol Extract ; Pedalium Murex Linn. ; Acute and Chronic Unpredictable Stress (CUS) ; Induced Models ; SD Rats. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Subramani R |
| Date Deposited: | 28 Jun 2019 17:16 |
| Last Modified: | 28 Jun 2019 17:16 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10659 |
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