Prabahar, T (2018) Bioprospective of Citrus Sinensis Linn. Leaves: Its Volatile Oil as a Weapon on Convulsion in Zebra Fish Larvae Seizure Model. Masters thesis, College of Pharmacy, Madurai Medical College, Madurai.
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Abstract
The dissertation highlights the pharmacognostical phytochemical and the anti-epileptic activity of the leaves of Citrus sinensis L which belonging to the family Rutaceae which should easily available variety in South Asian places , It has been used traditionally to treat ailments like constipation, cramps, colic, diarrhea, bronchitis, tuberculosis, cough, cold, obesity, menstrual disorder, angina, hypertension, anxiety, depression and stress. Leaf extracts of C. sinensis have been used in Nigerian local folk medicine to treat neurological disorders and to facilitate the digestion of food. It has also been used as an antidiabetic, antibacterial, antifungal, hypotensive, antioxidant, insect repellent, larvicidal, antiviral, uricosuric, anti-yeast, antihepatotoxic and antimutagenic agent due to the presence of copious oil. The morphological evaluvation showed the adherence of general characters to the family. • Detailed microscopical studies reveals the usual leaf characters like vascular bundles showing both xylem and phloem, anamocytic stomata, are seen only in lower epidermis. Two layers of palisade cells and secretory cavities are present. • Scanning electron microscope (SEM) shows presence of anamocytic stomata in the abaxial side of leaf. Stomata aperture and epidermal cells are clearly seen. • Petiole is winged, showing vascular bundle in the centre and secretory cavities. • Powder microcopy, microscopical analysis, vein islet number, vein termination number, stomatal number, stomatal index, palisade ratio, physiochemical parameters, ash values, extractive values, loss on drying were determined and represented. • Preliminary Phytochemical screening showed the presence of carbohytrates, proteins,amino acids, saponins, flavaonoids , triterpenes and volatile oils are present. • Identification of inorganic minerals of the leaves of C.sinensis by energy dispersive X-ray spectrometer(EDS) showed the presence of minerals. • Total flavanoids and total phenolic contents were determined and presented as 26.73 ± 1.10µg/g, 879.13 ± 0.92 µg /g. • GC-MS profile of the VOCSL showed the presence of α-Pinene, β-Pinene, Sabinene, Myrcene, Limonine, Terpinolene, Linalool and β-Elemene as major components along with many minor terpenes. • The 3 Rs (Reduction, Refinement, Replacement) of ethical principle was implemented that help to minimize harms to animals used in science. • In our investigation we used zebra fish larvae which is an emerging novel preclinical in vivo model that support rapid decision making in the early phases of drug discovery process. • We performed preliminary toxicological studies of VOCSL on the embryo and larvae of zebra fish and found no pronounced retardation in zf embryo development when exposed to normal concentrations (0.5 to 1.5µl/ml) which showed that VOCSL of leaf would produce no hazard to early life stages Danio rerio but standard toxin podophyllotoxin showed 100% mortality at 0.01µg/ml. • Larval toxicity study was carried out on zf larvae of 5dpf. From the experiment it was observed that there was no mortality in 0.5 and 0.75µl/ml concentrations. But 5% and 10% mortality was observed at 1 and 2 µl/ml concentrations respectively. No mortality was observed in the control. 100% mortality was observed in the standard podophyllotoxin at 0.5 µg/ml concentration. • We used Pentylene tetrazole (PTZ) to induce seizure like behaviour and increase in high speed moved distance travelled by PTZ treated zf larvae. • In our model VOCSL significantly decreases seizure like behaviour and also decrease in highspeed distance travelled at 0.5,1, 1.5µl/ml after treatment (p≤0.001) which is encouraging and comparable to the standard drug phenytoin. • The result exhibited concentration dependent inhibition of locomotor activity confirming its anticonvulsant characteristics. • We recommend further investigations in animal model and clinical trials to confirm the potential therapeutic effect.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No: 261620705 |
| Uncontrolled Keywords: | Bioprospective ; Citrus Sinensis Linn. Leaves ; Volatile Oil ; Weapon ; Convulsion ; Zebra Fish Larvae Seizure Model. |
| Subjects: | PHARMACY > Pharmacognosy |
| Depositing User: | Subramani R |
| Date Deposited: | 28 Jun 2019 15:12 |
| Last Modified: | 28 Jun 2019 15:12 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10646 |
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