Phytochemical and Pharmacological Assessment of Leucas Aspera (WILLD.) Link Whole Plant

Indira, S (2018) Phytochemical and Pharmacological Assessment of Leucas Aspera (WILLD.) Link Whole Plant. Masters thesis, J.K.K.Nattraja College of Pharmacy, Komarapalayam.


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Traditional Indian medicinal herbs have been used in the treatment of different diseases in the country for centuries. There have been claims that some traditional healers can successfully treat cancer using herbal drugs. The whole plant of Leucas aspera (Willd.) Link was selected and authenticated for the present study on the basis of ethanobotanical information. Literature survey revealed that not much work has done in this plant. So I felt it worthwhile to validate scientifically, the folkclaim for its therapeutic activity. The detailed preliminary phytochemical investigations proved its appropriate identification and rationalized its use as a drug of therapeutic importance. This plant have many phytoconstituents like flavonoids, alkaloids, saponins, tannins and so on. The phytoconstituents are found to possess pharmacological activities like antioxidant, antiulcer, antiinflammatory, antimicrobial activities. So it was planned to isolate active constituents from ethanol extract of whole plant of Leucas aspera (Willd.) Link. The present study concluded that the phytoconstituent was isolated from the whole plant of ethanol extract of Leucas aspera (Willd.) Link and characterized systematically with IR, H1 NMR and mass spectroscopy. The spectral datas of the isolated compound suggested that EELA I showed the structural similarities with Apigenin. Cancer is a complex multifactorial cell disease characterized by abnormal cellular proliferation. Cancer development is normally caused by oncogene, tumor suppressor gene, and microRNA gene alterations. It imposes a serious burden on the public health system, and its treatment and cure are scientifically challenging. Cancer is expected to claim nine million lives worldwide by 2015. Approximately 60% of anticancer agents are derived from medicinal plants and other natural resources; however, there are still a number of plants that have an anticancer potential but they have not yet been fully investigated. The MTT assay is used in screening the crude extracts as well as in the isolated compounds to assess the toxicity. It could also provide an indication of possible cytotoxic properties of the tested plant extracts. MTT assay is based on the reduction of MTT by mitochondrial dehydrogenase by purple formazan product. It is frequently used as an in vitro model system to measure cytotoxic effects of variety of toxic substances and plant extracts against cancer cell lines. Ethanol extract of Leucas aspera (Willd.) Link was screened for in vitro anticancer activitities in three different cell lines such as HeLa, Hep G2 and MCF-7 by MTT assay method. HepG2 produced more significant in vitro anticancer activity than other two cell lines. The present study concluded that the antioxidant potentials like flavonoids, saponins present in extract might be responsible for the anticancer activities. In conclusion, it was observed that the plant Leucas aspera (Willd.) Link contains a wide variety of secondary metabolites that hold strong antioxidant capacity based on the experiments performed which add scientific evidence to conduct further studies, investigate the lead compounds present in the plant, to evaluate its anticancer potential on in vivo animal models and put forward an attempt to carry out trials on human beings.

Item Type: Thesis (Masters)
Additional Information: Reg. No: 261615204
Uncontrolled Keywords: Leucas Aspera (WILLD.) ; Link Whole Plant ; Phytochemical and Pharmacological Assessment.
Subjects: PHARMACY > Pharmaceutical Chemistry
Depositing User: Subramani R
Date Deposited: 28 Jun 2019 02:18
Last Modified: 28 Jun 2019 02:18

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