Adaikkaladevi, A (2018) Preclinical Validation of Anti-Anxiety, Anti-Depressant and Anti-Cataleptic Activity of Classical Siddha Drug Sadhakuppai Chooranam in Animal Model. Masters thesis, Government Siddha Medical College, Chennai.
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Abstract
The trial drug Sadhakuppai Chooranam was selected from the Classical Siddha literature, “Agasthiyar Attavanai Vagadam” for the evaluation of safety and efficacy of the drug in “Paithiyam” (Neurosis). The ingredients of the test drug was identified and authenticated by Siddha experts. The drug was prepared as per the procedure and subjected to various studies to reveal its potency and effectiveness against the disease. Various analysis such as physicochemical, phytochemical, biochemical analysis, availability of bacterial and fungal load, instrumental analysis were made. The physical character of SKC shows good solubility and the pH of the trial drug is 5.65. Phytochemical screening test showed the presence of Alkaloids, Carbohydrates Glycosides, Saponins, Diterpenes, Flavonoids and Tannins are responsible for the anti-anxiety, antidepressant and anticataleptic activity. The HPLC results were made and it shows the prescence of Gallic acid, Flavanoids as Quercetin , Alkaloids, Tannic Acid. Biochemical analysis showed the presence of Calcium, Magnesium, Copper, Chloride and Phosphate. Calcium, Magnesium and Copper supports anti-anxiety and antidepressant activity of the trial drug (SKC). The availability of bacterial load in the (SKC) has been performed and the result shows presence of bacterial and fungal load within the normal limits of trial drug. The instrumental analysis FTIR showed the peak values present which are the functional groups responsible for its activity. SEM picture described its morphology and the particle size ranging from 10µm-20µm. The result of ICPMS shows the heavy metals like As, Hg, Cd and Pb were below detectable level. This reveals the safety of the drug. Toxicological study of both acute and sub-acute toxicity study were carried out in animal model Wistar albino rat according to the OECD guidelines. The test drug showed no acute toxicity as there was no mortality seen. The sub-acute toxicity after the repeated dose of 28 days was done The mortality, functional observations, haematological and biochemical investigations were made. There was no significant change seen in the normal values. Thus the toxicological study of the test drug greatly establishes the safety and gives the justification for long time administration. The pharmacological study was carried out in the animal model. Three activities were seen in the drug Sadhakuppai Chooranam. The activities were: 1. Anti-anxiety activity in Elevated plus maze. 2. Anti-depressant activity in Forced swimming test and Tail suspension test. 3. Anticataleptic activity in Haloperidol induced catalepsy in rats. Anti-anxiety activity was carried out in Elevated plus maze. The trial drug Sadhakuppai Chooranam - 200mg/kg b.w showed significant decrease in Anxiety condition. Thus this activity reveals the effect of the drug against Anxiety. Antidepressant activity of Sadhakuppai chooranam-200mg/kg b.w showed significant activity to reduce depression. Thus, this activity therapeutically effective in depressed condition. Anticataleptic activity of the test drug Sadhakuppai Chooranam -200mg/kg b.w was carried out in haloperidol induced catalepsy in rats. From the present study, it was concluded that the SKC extract has a marked Anti-cataleptict activity. Thus by scrutinizing all the above mentioned factors it is concluded that the trial drug Sadhakuppai Chooranam is a safe and a potent Anti-anxiety, Antidepressant and Anticataleptict drug. Modern medicine has its own limit in
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg. No: 321512102 |
| Uncontrolled Keywords: | Preclinical Validation ; Anti-Anxiety ; Anti-Depressant ; Anti-Cataleptic Activity ; Classical Siddha Drug ; Sadhakuppai Chooranam ; Animal Model. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Subramani R |
| Date Deposited: | 29 Dec 2018 13:37 |
| Last Modified: | 29 Dec 2018 13:37 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10232 |
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