Sumithra, M (2017) Preclinical Evaluation of the Possible Mechanisms of Anti-Hypertensive, Diuretic, Anti-oxidant Activities of Munthirikai Chooranam in Rodents. Masters thesis, Government Siddha Medical College, Chennai.
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Abstract
Munthirikai chooranam is a wonderful Siddha herbo mineral formulation which is indicated in Siddha texts as a best drug of choice to treat hypertension, vertigo etc., in order to scientifically evaluate this formulation, this drug undergoes physico chemical standardization, toxicity and pharmacological activity. 1. The organoleptic characters of the drug Munthirikai Chooranam was observed as brown, aromatic, astringent, fine powder, completely pass through sieve no 88 in nature. 2. The drug possess pH is 5.02, soluble in nature. 3. In phytochemical analysis the aqueous extract showed the presence of Alkaloid, Glycosides, Phenol, Triterpene, Flavonoid, Quinine, Protein in the sample MC. 4. The HPTLC analysis shows the fingerprints of the compounds present in the sample. 5. The presence of basic radicals such as Potassium, Calcium, Magnesium, Sodium, Iron, Zinc were identified in this Munthirikai Chooranam. 6. The presence of acid radicals such as Sulphate, Chloride, Nitrate were identified in this drug. 7. In microbial load analysis, the bacterial and fungal load found within the normal limits. This confirms that the drug is safe for clinical use. 8. The FTIR characterization of the Siddha medicinal compound Munthirikai Chooranam confirms that the presence of some functional groups such as alcohol, alkane, acid, alkyl halide, aromatic, amine, alkenes. 9. SEM analysis confirms the surface morphology of the drug. 10. The XRD analysis confirms the crystalline nature of the drug. 11. The results of ICPOES study shows the presence of Aluminium, Arsenic, Calcium, Cadmium, Copper, Iron, Mercury, Potassium, Magnesium, Sodium, Nickel, Lead, Phosphorus, Sulphur. The heavy metals such as Lead, Arsenic, Mercury, Cadimium were identified as within the WHO permissible limits. This clearly explains that the drug Munthirikai Chooranam is safe for therapeutic usage. 12. There is no toxicity findings at the acute toxicity level determination. Based on OECD 423 the trail drug Munthirigai Chooranam is considered non toxic up to the dose of 2000mg/kg. 13. In 28 days repeated dose oral toxicity study in rats to evaluate the toxicity findings of the drug, the haematological, biochemical parameters, body weight are all remains in the normal level. After the drug administration, the histopathological findings were analyzed and those also revealed no abnormal findings. This indicates that there is no observed level of toxicity findings at these fixed dose levels of low dose 100 mg and high dose 200 mg. Hence the study confirms that there is no observed level of toxicity in the 28 days repeated oral drug administration. From toxicological study results the drug is free from toxic effects for human use at therapeutic dose level. 14. In SHR rats, the drug Munthirikai Chooranam reduces the blood pressure and heart rate in a dose dependant manner. This clearly explains that this Siddha medicinal compound having strong anti-hypertensive potential. 15. In DPPH assay, the drug Munthirikai Chooranam possess potent antioxidant property comparably higher than the synthetic antioxidant BHT. This confirms the antioxidant nature of the drug. These research works confirms that the drug is safe for clinical use. And the studies possess that the Siddha herbo mineral formulation Munthirikai Chooranam has anti hypertensive, diuretic and anti oxidant property.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Preclinical Evaluation ; Possible Mechanisms ; Anti-Hypertensive ; Diuretic ; Anti-oxidant Activities ; Munthirikai Chooranam ; Rodents. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Subramani R |
| Date Deposited: | 29 Dec 2018 13:37 |
| Last Modified: | 29 Dec 2018 13:37 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10230 |
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