Kanimozhi, C (2017) Preclinical Validation of Anti-Anxiety, Anti-Depressant and Anti-Convulsant activity of classical Siddha drug Kandathirika Chooranam in Animal Model. Masters thesis, Government Siddha Medical College, Chennai.
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Abstract
The trial drug Kandathirika Chooranam was selected from the Classical Siddha literature, “Agasthiyar Vaithiya Sinthamani Venba 4000 Enum Mani 4000”- Part I for the evaluation of safety and efficacy of the drug in “Paithiyam” (Neurosis). The ingredients of the test drug was identified and authenticated by Siddha experts. The drug was prepared as per the procedure and subjected to various studies to reveal its potency and effectiveness against the disease. Various analy sis such as physicochemical, phytochemical, biochemical analysis, availability of bacterial and fungal load, instrumental analysis were made. The physical character of KCM shows good solubility and the pH of the trial drug is 3.83 Phytochemical screening test showed the presence of Alkaloids, Glycosides, Phenol, Triterpenes, Flavonoids, Tannins and Protein. Flavonoids, Glycosides, Tannins are responsible for the anti-anxiety and antidepressant activity. The TLC/HPTLC finger prints were made and it shows 12peaks. In which the four major peaks denotes presence of phytochemicals and Rf value of the trial drug supports the better standardisation of the trial drug (KCM). Biochemical analysis showed the presence of Calcium, Magnesium, Copper, Chloride and Phosphate. Calcium, Magnesium and Copper supports anti-anxiety and antidepressant activity of the trial drug (KCM). The availability of bacterial load in the KCM has been performed and the result shows presence of bacterial and fungal load within the normal limits of trial drug. The instrumental analysis FTIR showed the peak values present which are the functional groups responsible for its activity. SEM picture described its morphology and the particle size ranging from 100nm-1µm.The result of ICPOES shows presence of Ca, K, Mg, Na, P and S has physiologically important and the heavy metals like As, Cd, Hg, Pb and Ni were below detectable level. This reveals the safety of the drug. Toxicological study of both acute and sub-acute toxicity study were carried out in animal model Wistar albino rat according to the OECD guidelines. The test drug showed no acute toxicity as there was no mortality seen. The sub-acute toxicity after the repeated dose of 28 days was done. The mortality, functional observations, haematological and biochemical investigations were made. There was no significant change seen in the normal values. Thus the toxicological study of the test drug greatly establishes the safety and gives the justification for long time administration. The pharmacological study was carried out in the animal model. Three activities were seen in the drug Kandathirika Chooranam. The activities were: 1. Anti-anxiety activity in Elevated plus maze 2. Anti-depressant activity in Forced swimming test and Tail suspension test. 3. Anticonvulsant activity –PTZ induced seizures in mice. Anti-anxiety activity was carried out in Elevated plus maze. The trial drug Kandathirika Chooranam-400mg/kg b.w showed significant decrease in Anxiety condition. Thus this activity reveals the effect of the drug against Anxiety. Antidepressant activity of Kandathirika chooranam-200mg/kg b.w showed significant activity to reduce depression. Thus this activity therapeutically effective in depressed condition. Anticonvulsant activity of the test drug Kandathirika Chooranam was carried out in PTZ induced seizures in mice. From the present study, it was concluded that the KCM extract has a marked Anti-convulsant activity at higher concentrations. Thus by scrutinizing all the above mentioned factors it is concluded that the trial drug Kandathirika Chooranam is a safe and a potent Anti-anxiety, Antidepressant and Anticonvulsant drug. Modern medicine has its own limit in treating Neurosis.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg No:321412106 |
| Uncontrolled Keywords: | Preclinical Validation ; Anti-Anxiety ; Anti-Depressant ; Anti-Convulsant activity ; classical Siddha drug ; Kandathirika Chooranam ; Animal Model. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Subramani R |
| Date Deposited: | 29 Dec 2018 13:37 |
| Last Modified: | 29 Dec 2018 13:37 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/10229 |
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